Medical academic journalMedical academic journalМедицинский академический журнал1608-41012687-1378Eco-Vector6289210.17816/MAJ62892Original study articlesОригинальные исследованияResearch ArticleMechanisms of the influence of adiponectin on apolipoproteins A-1 and B production by human hepatocytesМеханизмы влияния адипонектина на продукцию аполипопротеинов А-1 и B гепатоцитами человекаhttps://orcid.org/0000-0002-5321-88349303-9445TanyanskiyDmitriy A.ТанянскийДмитрий Андреевич
Russian Federation

MD, PhD (Medicine), Head of Laboratory of Lipoproteins, Department of Biochemistry

канд. мед. наук, заведующий лабораторией липопротеинов им. акад. РАМН А.Н. Климова отдела биохимии

dmitry.athero@gmail.comhttps://orcid.org/0000-0001-5147-47491625-0496DizheElla B.ДижеЭлла Борисовна
Russian Federation

MD, PhD (Biology), Leading Researcher, Department of Biochemistry

канд. биол. наук, ведущий научный сотрудник отдела биохимии

dizhe@iem.sp.ruOleinikovaGalina N.ОлейниковаГалина Николаевна
Russian Federation

Research Assistant, Department of Biochemistry

лаборант-исследователь отдела биохимии

galina@iem.sp.ru5428-6800ShavvaVladimir S.ШавваВладимир Станиславович
Russian Federation

MD, PhD (Biology), Senior Researcher, Department of Biochemistry

канд. биол. наук, старший научный сотрудник отдела биохимии

vssreinard.fox@gmail.comhttps://orcid.org/0000-0003-1613-06547496-1449DenisenkoAleksandr D.ДенисенкоАлександр Дорофеевич
Russian Federation

MD, PhD, DSc (Medicine), Professor, Head of Department of Biochemistry

д-р мед. наук, профессор, заведующий отделом биохимии

add@iem.sp.ruInstitute of Experimental MedicineФедеральное государственное бюджетное научное учреждение «Институт экспериментальной медицины»230320211006202121139451003202123032021Copyright ©; 2021, Tanyanskiy D.A., Dizhe E.B., Oleinikova G.N., Shavva V.S., Denisenko A.D.Copyright ©; 2021, Танянский Д.А., Диже Э.Б., Олейникова Г.Н., Шавва В.С., Денисенко А.Д.2021Tanyanskiy D.A., Dizhe E.B., Oleinikova G.N., Shavva V.S., Denisenko A.D.Танянский Д.А., Диже Э.Б., Олейникова Г.Н., Шавва В.С., Денисенко А.Д.https://creativecommons.org/licenses/by-nc-nd/4.0https://journals.eco-vector.com/MAJ/article/view/62892

The aim of the study was to find out the mechanisms of the adiponectin effect on apolipoproteins (apo) A-1 and B production by human hepatocytes.

Materials and methods. The study was performed on the human hepatoma cell line HepG2. The expression of the apoA-1 gene was evaluated at the mRNA level by quantitative PCR with reverse transcription, and the production of apoB – by ELISA method. The activity of lipogenesis was assessed by the inclusion of labeled 14C-acetate in triglycerides, as well as by mRNA expression of lipogenesis genes, and by the estimation of total triglycerides content in cells. To determine the involvement of signaling pathways, the RNA interference method was used.

Results. Knockdown of genes, coding the specific receptors, AMP-activated protein kinase, and its regulated transcription factors inhibited adiponectin-dependent stimulation of apoA-1 gene expression in hepatocytes. Adiponectin had no effect on lipogenesis and apoB production under basal conditions, but suppressed these processes induced by the addition of oleate.

Conclusion. Adiponectin stimulates the production of apoA-1 in hepatocytes by inducing the transcription of the apoA-1 gene and suppresses the secretion of apoB by affecting lipogenesis. These effects may underlie the effect of adiponectin on lipoproteins metabolism.

Цель исследования — выяснить механизмы влияния адипонектина на продукцию аполипопротеинов (апо) А-1 и В гепатоцитами человека.

Материалы и методы. Исследование проводили на клетках линии гепатомы человека HepG2. Экспрессию гена apoA-1 оценивали на уровне мРНК методом количественной полимеразной цепной реакции с обратной транскрипцией, продукцию апоВ — методом иммуноферментного анализа. Активность липогенеза определяли по включению меченого 14С-ацетата в триглицериды, по экспрессии генов липогенеза на уровне мРНК и по общему содержанию триглицеридов в клетках. Для выяснения участия сигнальных путей использовали метод РНК-интерференции.

Результаты. Нокдаун генов специфических рецепторов, АМФ-активируемой протеинкиназы и регулируемых ею факторов транскрипции приводил к отмене адипонектин-зависимой стимуляции экспрессии гена apoA-1 в гепатоцитах. Адипонектин не влиял на липогенез и продукцию апоВ в базальных условиях, но при этом подавлял данные процессы, индуцированные добавлением олеата.

Заключение. Адипонектин стимулирует продукцию апоА-1 в гепатоцитах путем индукции транскрипции гена apoA-1 и подавляет секрецию данными клетками апоВ посредством влияния на липогенез. Указанные воздействия могут лежать в основе влияния адипонектина на обмен липопротеинов.

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