Medical academic journalMedical academic journal1608-41012687-1378Eco-Vector7629010.17816/MAJ76290Conference Report, Theses of ReportProtective role of silver nanoparticles in influenza infectionAl FarroukhMohammad<p>Postgraduate student</p>mouhammad1farroukh@gmail.comhttps://orcid.org/0000-0003-0017-4126SkomorokhovaEkaterina A.<p>PhD (Biol.), Researcher</p>katjaskom@yandex.ruhttps://orcid.org/0000-0001-6018-2190MagazenkovaDaria N.<p>engineer</p>magdash@mail.ruhttps://orcid.org/0000-0003-1959-7110KiselevaIrina V.<p>Dr. Sci. (Biol.), Professor, Laboratory Head</p>irina.v.kiseleva@mail.ruhttps://orcid.org/0000-0002-3892-9873Institute of Experimental MedicineSaint Petersburg State UniversityITMO University0612202121381841907202106082021Copyright © 2021, Al Farroukh M., Skomorokhova E.A., Magazenkova D.N., Kiseleva I.V.2021<p><strong><em>BACKGROUND:</em></strong> The present study assesses copper metabolism of the host organism as a target of antiviral strategy, basing on the virocell concept. This concept suggests that the targets for suppressing viral reproduction can be found in the hosts metabolism.</p>
<p><strong><em>AIM:</em></strong> Evaluation of the effect of copper status indicators on influenza infection in mice.</p>
<p><strong><em>MATERIALS AND METHODS:</em></strong> Silver nanoparticles (AgNPs) were used as a specific active agent because they reduce the level of holo-ceruloplasmin, the main extracellular cuproenzyme. The mouse model of influenza virus A infection was used with two doses: 1 LD<sub>50</sub> and 10 LD<sub>50</sub>. The following treatment regimens were used: mice were pretreated four days before infection and then every day during infection development until the end of the experiment (day 14).</p>
<p><strong><em>RESULTS:</em></strong> The mice treated with AgNPs demonstrated significantly lower mortality, the protection index reached 6070% at the end of the experiment, and mean lifespan was prolonged. In addition, the treatment of the animals with AgNPs resulted in normalization of the weight dynamics. Despite the amelioration of the infection, AgNPs treatment did not influence influenza virus replication.</p>
<p><strong><em>CONCLUSIONS:</em></strong> This study provides support for the view that silver nanoparticles could be used as protection against influenza.</p>ceruloplasmincopper statussilver nanoparticlesinfluenzainfluenza virus replicationprophylaxis and treatmentцерулоплазминстатус мединаночастицы серебрагриппрепликация вируса гриппапрофилактика и лечение[Up to 650 000 people die of respiratory diseases linked to seasonal flu each year [Internet]. WHO. 2017. Available from: http://www.who.int/mediacentre/news/releases/2017/seasonal-flu/en/. Accessed: June 2, 2021.][Forterre P. The virocell concept and environmental microbiology. ISME J. 2013;7(2):233–236. DOI: 10.1038/ismej.2012.110][Xiang D, Duan W, Shigdar S, et al. Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in vitro and in vivo. International Journal of Nanomedicine. 2013;8:4103–4113. DOI: 10.2147/ijn.S53622][Xiang DX, Chen Q, Pang, L, Zheng, CL. Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro. J Virol Methods. 2011;178(1–2):137–142. DOI: 10.1016/j.jviromet.2011.09.003][Mehrbod P, Motamed N, Tabatabaian M, et al. In vitro antiviral effect of “nanosilver” on influenza virus. DARU Journal of Pharmaceutical Sciences. 2009;17(2):88–93.]