NEW OPPORTUNITIES IN PREDICTION OF RISK FOR DEVELOPMENT OF PROFESSIONAL ALLERGIC DISEASES

Aim. To work out complex of methods for assessment of risk for development of professional allergic diseases. Materials and methods. Gene polymorphism was analyzed using the method of multiplex polymerase chain reaction. Genomic DNA was extracted from peripheral blood with the method of phenolic-chloroform extraction. To control correspondence of the observed distribution of genotype frequencies to theoretically expected balanced distribution according to Hardy-Weinberg law, χ 2 criterion was applied. The integrated risk index was calculated for a concrete person according to prognostic map taking into account gradations for each risk factor by means of summing the scores and comparing the obtained values with the risk scale. Results. New professional allergic disease risk markers were found. In presence of genotype CC ( OR =3,23) and allele C ( OR =2,07) of polymorphic graft rs2069812 of gene IL5, risk for development of PBA increased by 3 and 2 times, respectively. In presence of allele T of polymorphic graft rs3939286 of gene IL33, risk for development of allergic dermatitis increased by 1,5 times ( OR =1,56). Elevated risk for development of allergic rhinitis was determined by genotype CT and allele T of polymorphic graft rs3939286 of gene IL33 ( OR =1,35, OR =1,45, respectively). Integrated risk index (P) for a concrete person was calculated individually according to prognostic map taking into account presence of gradations for each risk factor by means of summing the scores and comparing the obtained value with the risk scale: P = Σ scores. Risk scale was calculated by determination of risk range. The border of minimum risk is equal to the sum of minimum values of scores for all factor gradations P min= Σ scores corresponding to R min=14. The border of maximum risk is equal to the sum of maximum values scores for all factor gradations P max= Σ scores corresponding to R max=23. Conclusions. The offered methods permit to assess the risks for development of professional diseases of allergic etiology with high reliability.

Occupational diseases of allergic etiology, risks, genetic markers