Microcirculation parameters in rats exposed to biotechnological preparations of Cordyceps militaris fungi



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Abstract

BACKGROUND: Our previous studies demonstrated that administration of Cordyceps militaris (L.) Fr. biomass and culture fluid extracts resulted in significant changes in blood pressure and heart rate in rats. However, the effect of these fungal biotechnological products on microcirculation processes has not been studied.

BACKGROUND: Our previous studies demonstrated that administration of Cordyceps militaris (L.) Fr. biomass and culture fluid extracts resulted in significant changes in blood pressure and heart rate in rats. However, the effect of these fungal biotechnological products on microcirculation processes has not been studied.

AIM: To determine changes in microcirculation parameters in rats exposed to Cordyceps militaris (L.) Fr. mushroom extracts.

METHODS: Group 1 served as a control group, Group 2 (ECG) received 0.5 ml of C. militaris culture fluid extract, and Group 3 (EBM) received 0.5 ml of Cordyceps militaris (L.) Fr. biomass extract. Microcirculation was studied using laser Doppler flowmetry using a Lazma-MC laser blood flow analyzer (version 2, manufactured by Lazma Research and Production Enterprise, Russia). Both non-oscillatory and oscillatory parameters of tissue microhemodynamics were recorded.

RESULTS: Intraperitoneal administration of an extract of the in vitro-obtained mycelial biomass (EBM) of Cordyceps militaris (L.) Fr. resulted in a 30.3% increase in tissue perfusion (p=0.0026) due to increased endothelial metabolic activity, decreased sympathetic adrenergic pressor effects, and decreased precapillary sphincter and precapillary metarteriole tone due to the development of Ca2+-dependent muscle relaxation. This was evidenced by significant increases in all oscillatory and non-oscillatory parameters of microcirculation.

With the administration of ECG Cordyceps militaris (L.) Fr., perfusion increased by 32.6% (p=0.0016), Ae - by 55% (p=0.0072), An - by 77.9% (p=0.0121), Am by - 82.1% (p=0.0099), and Ad - by 45.5% (p=0.0370) compared to those in the control group of animals, indicating the development of vasodilation.

CONCLUSIONS: Thus, as analysis of the study results showed, the administration of C. militaris ECF and EBM to animals has a beneficial effect. led to an increase in endothelium-dependent vasodilation, a decrease in peripheral resistance, an increase in blood flow into the nutritional microvascular bed, and an improvement in venous outflow.

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About the authors

Marina Ravaeva

FSAEI HE “V.I. Vernadsky Crimean Federal University”

Email: ravaevam@yandex.ru
ORCID iD: 0000-0002-6081-1628
SPIN-code: 2398-3901
Scopus Author ID: 6505646236

Candidate of Biological Sciences, Associate Professor of the Department of Human and Animal Physiology and Biophysics

Russian Federation, 4 Akademika Vernadckogo av., Simferopol, 295007, Russia

Maria Viktorovna Nagorskaya

Federal State Autonomous Educational Institution of Higher Education “V.I. Vernadsky Crimean Federal University

Email: zaygolnikova@mail.ru
ORCID iD: 0009-0002-0528-2791
SPIN-code: 9067-5216

postgraduate student at the Department of Human and Animal Physiology and Biophysics.; Institute of Biochemical Technologies, Ecology and Pharmacy of the V.I. Vernadsky Crimean Federal University

Russian Federation, 4 Akademika Vernadckogo av., Simferopol, 295007, Russia;

Andrey Ivanovich Sidyakin

Federal State Autonomous Educational Institution of Higher Education “V.I. Vernadsky Crimean Federal University”

Email: acid2302@mail.ru
ORCID iD: 0000-0002-5125-7514
SPIN-code: 8216-8455
Scopus Author ID: 57298469300

Cand. Sci. Biol, Associate Professor of the Department of Botany and Plant Physiology; Institute of Biochemical Technologies, Ecology and Pharmacy of the V.I. Vernadsky Crimean Federal University

Russian Federation, 4 Akademika Vernadckogo av., Simferopol, 295007, Russia,

Elena Nikolaevna Chuyan

Federal State Autonomous Educational Institution of Higher Education “V.I. Vernadsky Crimean Federal University”

Email: elena-chuyan@rambler.ru
ORCID iD: 0000-0001-6240-2732
SPIN-code: 8373-3871
Scopus Author ID: 9436061900

Doctor of Biological Sciences, Professor, Head of the Department of Human and Animal Physiology and Biophysics of the Institute of Biochemical Technologies, Ecology and Pharmacy of the Federal State Autonomous Educational Institution of Higher Education “V.I. Vernadsky Crimean Federal University”

Russian Federation, 4 Akademika Vernadckogo av., Simferopol, 295007, Russia,

Tatyana Valentinovna Zayachnikova

Federal State Autonomous Educational Institution of Higher Education “V.I. Vernadsky Crimean Federal University”

Email: tanyaz75@mail.ru
ORCID iD: 0009-0009-5860-5399
SPIN-code: 7777-1441
Scopus Author ID: 14057382100

Cand. Sci. Biol.; Department of Human and Animal Physiology and Biophysics Institute of Biochemical Technologies, Ecology and Pharmacy of the V.I. Vernadsky Crimean Federal University

Russian Federation, 4 Akademika Vernadckogo av., Simferopol, 295007, Russia;

Pavel Aleksandrovich Galenko-Yaroshevskii

Kuban State Medical University, Krasnodar, Russia

Email: galenko.yarochevsky@gmail.com
ORCID iD: 0000-0003-0873-284X
SPIN-code: 1575-6129
Scopus Author ID: 6603110151

Dr. Sci. Biol., Corresponding Member of the Russian Academy of Sciences; Kuban State Medical University, Krasnodar

Russian Federation, 350063, Russian Federation, Krasnodar Territory, Krasnodar, 4 Mitrofan Sedin Street

Andrey Vladimirovich Zadorozhniy

Rostov State Medical University, Rostov-on-Don, Russia

Author for correspondence.
Email: stomvr1@gmail.com
ORCID iD: 0000-0001-9552-8542
SPIN-code: 8547-1287
Scopus Author ID: 915098

PhD in Medical Sciences, Associate Professor, Head of the Department of Dentistry No.4, Rostov State Medical University, Rostov-on-Don, Russia

Russian Federation, 344022, Russia; Rostov Region, Rostov-on-Don, Nakhichevansky Lane, 29

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