Reviews on Clinical Pharmacology and Drug TherapyReviews on Clinical Pharmacology and Drug Therapy1683-41002542-1875Eco-Vector617510.17816/RCF15153-57Research ArticleThe influence of phlomoides tuberosa extract on structure of spleen in immunosupressive miceTsyrenovaDarima Z.<p>post-graduate student, Laboratory of Experimental Pharmacology</p>dtsyrenova@mail.ruGulyaevSergey M.<p>PhD, Senior staff scientist, Laboratory of Safety of Biologically Active Substances</p>s-gulyaev@inbox.ruKhobrakovaValentina B.<p>Dr, Senior staff scientist, Laboratory of Experimental Pharmacology</p>val0808@mail.ruInstitute of General and Experimental Biology SB RAS15032017151535717042017Copyright © 2017, Tsyrenova D.Z., Gulyaev S.M., Khobrakova V.B.2017<p><strong>Background</strong>. The immune system is extremely sensitive to adverse external and internal factors. In this context, a correction of immunosuppressive states of immune organs by means of complex and safe means, such as the extraction of medicinal plants is required. In this regard, plants containing phenylpropanoids are of interest. It was shown that the extract from Phlomoides tuberosa L. Moench showed immunostimulatory activity due to the content phenylpropanoids.</p>
<p><strong>Purpose</strong>. The present study was aimed at the estimation of the effect of P. tuberosa extract оn the spleen structure using experimental azathioprine immunosupression model.</p>
<p><strong>Materials and methods.</strong> The experiments were carried out on CBA male mice, 20-22 g of weight. Immune deficiency was modeled by the intragastrical introduction of azathioprine in the dose 50 mg/kg once a day for 5 days. The experimantal group of animals received the tested remedy once a day intragastrically in dose 1 mg/kg for 14 days against the background of azathioprine immunosupression. The intact group received the purified water according to the analogous scheme. Data were obtained using histological, morphometric and statistical analysis.</p>
<p><strong>Results</strong>. The results have shown that azathioprine caused disruptive changes in the structure of the lymphoid tissue of the spleen: area of white pulp and the thickness of the periarterial lymphoid sheath (PALS) were equally less in 1.7 times, the diameters of the lymphoid nodules and germinative centers were lower, respectively in 2.6 and 1.7 times, compared with a negative control. The course administration of the test remedy to animals was significantly reduced suppressive effect of cytostatic azathioprine: area of white pulp and the thickness PALS spleens of mice treated with the extract were 1.5 and 1.4 times the diameters of lymphoid nodules and germinative centers was 2.1 times and 1.2 times more positive control.</p>
<p><strong>Conclusion</strong>. Thus, the data obtained allow to conclude that the dry extract P. tuberosa has the marked immune modulating activity that substantiates the expediency of its further study in order to create new effective immune modulators.</p>immunityimmunosuppressionazathioprinePhlomoides tuberosa extractimmunomodulating activitymicespleenmorphologyиммунитетиммуносупрессияазатиопринэкстракт Phlomoides tuberosaиммуномодулирующая активностьмышиселезенкаморфология[1. Автандилов Г.Г. Основы количественной патологической анатомии: учебное пособие. – М.: Медицина, 2002. [Bases of quantitative pathological anatomy: a textbook. Moscow: Meditsina; 2002. (In Russ.)]][2. Гуляев С.М., Цыренова Д.З., Хобракова В.Б. Влияние Phlomoides tuberosa на структуру тимуса мышей при иммуносупрессии // Вопросы биологической, медицинской и фармацевтической химии. – 2016. – № 12. – С. 41–44. [Gulyaev SM, Tsyrenova DZ, Khobrakova VB. [The influence of Phlomoides tuberosa extract on morhofunctional state of thymus in immunosupressive mice. Voprosy biologicheskoy, meditsinskoy i farma tsevticheskoy khimii. 2016;(12):41-44. (In Russ.)]][3. Кащенко С.А., Золотаревская М.В. Изменения морфометрических показателей белой пульпы селезенки крыс под воздействием иммунотропных препаратов // Укр. мед. альм. – 2011. – Т. 14. – № 5. – С. 74–77. [Kashchenko SA, Zolotarevskaya MV. The changes of morphometric indexes of white pulp of rats’ spleen under influence of immunomodulate drugs. Ukr med al’m. 2011;14(5):74-77. (In Russ.)]][4. Куркин В.А., Авдеева Е.В., Суворова А.В., Дубищев А.В. Актуальные проблемы и перспективы развития фитофармакологии и фитотерапии // Мед. альманах. – 2008. – № 4. – С. 41–44. [Kurkin VA, Avdeeva EV, Suvorova AV, Dubishchev AV. [Acute problems and development prospects of phytopharmacology and phytotherapy. Med. al’manakh. 2008;(4):41-44. (In Russ.)]][5. Куркин В.А., Запесочная Г.Г., Ежков В.Н. Фенилпропаноиды лекарственных растений. – Самара: Офорт, 2005. [Kurkin VA, Zapesochnaya GG, Ezhkov VN. Phenylpropanoids from medicinal plants. Samara: Ofort; 2005. (In Russ.)]][6. Лазарева Д.Н., Алехин Е.К. Стимуляторы иммунитета. – М.: Медицина; 1985. [Lazareva DN, Alekhin EK. Immune stimulants. Moscow: Meditsina; 1985. (In Russ.)]][7. Лимонов В.Л., Шурлыгина А.В., Робинсон М.В., и др. Морфологическое исследование почек и селезенки у мышей с иммунокомплексным гломерулонефритом, интактных и на фоне иммуносупрессивной терапии // Бюллетень СО РАМН. – 2005. – № 2. – С. 50–54. [Limo nov VL, Shurlygina AV, Robinson MV, et al. [Morphological re search of the kidneys and spleen in mice with immune complex glomerulonephritis, intact and in immunosuppression therapy. Byulleten’ SO RAMN. 2005;(2):50-54. (In Russ.)]][8. Меркулов Г.А. Курс патологогистологической техники. – Л.: Медицина, 1969. [Merkulov GA. Course of pathologic-histological technology. Leningrad: Meditsina; 1969. (In Russ.)]][9. Сепиашвили Р.И. От иммунотерапии к персонализированной таргетной иммуномодулирующей терапии и иммунореабилитации // Аллергология и иммунология. – 2015. – Т. 16. – № 4. – С. 323–327. [Sepiashvili RI. From immunotherapy to personalized targeted immunomodulating therapy and immunorehabilitation. Aller gologiya i immunologiya. 2015;16(4):323-327. (In Russ.)]][10. Хайдав Ц., Меньшикова Т.А. Лекарственные растения в монгольской медицине: Историко-медицинские исследования. – Улаанбаатар, 1985. [Khaydav Ts, Men’shikova TA. Medicinal plants in Mongolia: historical and medical research. Ulaanbaatar; 1985. (In Russ.)]][11. Цыренова Д.З., Хобракова В.Б. Влияние сухого экстракта зопника клубненосного на фагоцитарную активность макрофагов при экспериментальной иммуносупрессии: Материалы международного форума «Клиническая иммунология и аллергология — междисциплинарные проблемы». – Казань, 2014. – С. 276–277. [Tsyrenova DZ, Khobrakova VB. [The influence of the dry extract from Phlomis tuberosa (L.) Moench on the phagocytic activity of macrophages in experimental immunosupression. (Conference proceedings) Materialy mezhdunarodnogo foruma “Klinicheskaya immunologiya i allergologiya – mezhdistsiplinarnye problem”. Kazan’; 2014. P. 276-277. (In Russ.)]][12. Шевцов А.Р., Головнёв В.А., Голубева И.А. Морфология селезенки в норме, при моделировании синдрома длительного сдавления и в условиях применения полифенолов манжетки обыкновенной // Вестник НГУ. Серия «Биология, клиническая медицина». – 2006. – Т. 4. – № 3. – С. 62–5. [Shevtsov AR, Golovnev VA, Golubeva IA. [The spleen morphology in normal state, during modelling of compression syndrome and under application of Alchemilla vulgaris polyphenols. Vestnik NGU. Seriya «Biologiya, klinicheskaya meditsina». 2006;4(3):62-5. (In Russ.)]][13. Ahmad W, Jantan I, Kumolosasi E, Abbas Bukhari SN. Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats. Drug Des Devel Ther. 2015;9:2961-73.][14. Sultan MT, Butt MS, Qayyum MM, Suleria HA. Immunity: plants as effective mediators. Crit Rev Food Sci Nutr. 2014;54(10):1298-308. doi: 10.1080/10408398.2011.633249.][15. Harmatha J, Zidek Z, Kmonickova E, Smidrkal J. Immunobiological properties of selected natural and chemically modified phenylpropanoids. Interdisc Toxicol. 2011;4(1):5-10. doi: 10.2478/v10102-011-0002-1.]