EFFECTS OF GHRELIN AND ITS ANTAGONIST [D-LYS3]-GHRP-6 ON THE CONDITIONED REACTION OF PLACE PREFERENCE IN ALCOHOLIZED CHRONICALLY RATS



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Abstract

The effects of ghrelin and its antagonist [D-Lys3]-GHRP-6 on the formation, storage and extinction of the conditioned reaction of place preference (CRPP) of ethanol (0.5 g/kg ip) in a 2-chambers apparatus were studied in rats alcoholized with 15% ethanol solution during 6 months. [D-Lys3]-GHRP-6, an antagonist of the ghrelin receptors, administered intranasally in the dose of 10 µg in 20 µl, blocked the CRPP acquisition that was performed by deterioration of staying time in the preferable chamber associated with ethanol by 29.5%. After extinction of CRPP both ghrelin (20 µg in 20 µl intranasally) and [D-Lys3]-GHRP-6 (10 µg in 20 µl) blocked the recovery (or expression) of CRPP of ethanol by 30% in average. It is concluded that ghrelin system participates in the mechanisms of conditioned reinforcement (2 tables, bibliography: 21 refs).

About the authors

Petr D. Shabanov

S. M. Kirov Military Medical Academy the Russian Defense Ministry

Author for correspondence.
Saint Petersburg

Vitaliy I. Morozov

Institute of Experimental Medicine

Saint Petersburg

Andrey A. Lebedev

Institute of Experimental Medicine

Saint Petersburg

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