Russian Military Medical Academy ReportsRussian Military Medical Academy Reports2713-23152713-2323Eco-Vector10921110.17816/rmmar109211Research ArticleThe effectiveness of the use of mesenchymal stromal cells for the treatment of lacerated wounds under conditions of hypothermia and hypoxiaVolkovaMarina V.<p>Junior Research Fellow</p>biotech.volkova@list.ruhttps://orcid.org/0000-0001-5966-3026BoyarintsevValery V.marinarage@mail.ruhttps://orcid.org/0000-0001-9707-3262TrofimenkoAlexander V.<p>M.D., Ph.D. (Medicine), Associate Professor, Head of Laboratory</p>trofimenko.av@mipt.ruRybalkinSergey P.<p>Ph.D. (Biology), director</p>rybalkin-sp@yandex.ruhttps://orcid.org/0000-0002-2933-5758KovalevaElena V.<p>Senior Researcher</p>e-kovaleva@yandex.ruBiryukovStanislav A.<p>D.Sc. (Physics and Mathematics), Senior Researcher</p>biryukov.sa@mipt.ruFil'kovGleb I.<p>Researcher</p>filcom.gl@gmail.comDurymanovMichail O.<p>Ph.D. (Biology), Deputy Head of Laboratory</p>durymanov.mo@mipt.ruMoscow Institute of Physics and Technology (National Research University)Institute of Immunology of the Federal Medical and Biological Agency of Russia, Research Center for Toxicology and Hygienic Regulation of Biological Products191020224132612680607202214072022Copyright © 2022, Eco-Vector2022<p><strong><em>BACKGROUND</em></strong>: Patients Arctic conditions complicate the pathogenesis of various skin and soft tissue injuries. For the treatment of these diseases, the possibility of using multipotent mesenchymal stromal cells, which promote the proliferation of granular tissue cells, angiogenesis, and reduce the duration of the inflammatory phase during wound healing due to the secretion of cytokines and growth factors, is being considered.</p>
<p><strong><em>AIM</em></strong>: In order to evaluate the effectiveness of cell therapy, experimental studies were carried out on the model of a lacerated wound in rats under conditions of hypoxia and hypothermia.</p>
<p><strong><em>MATERIALS AND METHODS</em></strong>: The animals were kept in a climate chamber (15% oxygen, 4C) for 48 hours. Injury was applied 24 hours after placement in controlled conditions. The introduction of stem cells was carried out a day after the wound was applied. Mesenchymal stromal cells obtained from the red bone marrow of Wistar rats were used for injection. The cell culture used had an immunophenotype corresponding to stem cells and had the ability to differentiate in the osteogenic, chondrogenic and adipogenic directions. During the study, the degree of inflammatory reaction in injured tissues and the presence of possible pathological discharges from the wound canal were assessed in rats, and the thickness of the injured paw was measured.</p>
<p><strong><em>RESULTS</em></strong>: The stimulating effect of the suspension of mesenchymal stem cells on the dynamics of reducing the edema of the injured hip by 10% was established compared to the control group. To describe the process of inflammation, a histological analysis was performed on the 6<sup>th</sup> and 21<sup>st</sup> days after the wound was applied. On the 6<sup>th</sup> day of the study, a weak infiltration of lymphocytes in the muscle tissue was noted in rats that were injected with MMSC, which may indicate an earlier transition of the wound process to the proliferative phase.</p>
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