Russian Military Medical Academy ReportsRussian Military Medical Academy Reports2713-23152713-2323Eco-Vector4331010.17816/rmmar43310Research ArticleINFLUENCE FEATURES OF EXOMETABOLITES OF CAT TAPEWORM CYSTICERCUS ON THE PROLIFERATIVE ACTIVITY OF L929 CELLSAdoevaE. Ya-BityutskyL. M-ZvezdinV. V-S.M. Kirov Military Medical Academy of the Ministry of Defense of the Russian Federation151220202S171028082020Copyright © 2020, Adoeva E.Y., Bityutsky L.M., Zvezdin V.V.2020Tissue parasites in the host organism cause a number of local and general changes aimed at ensuring a long-term existence. They secrete a complex of biologically active substances that stimulate the proliferation of various cells. This work is a fragment of a comprehensive study of relationships in host-parasite systems in tissue larval helminthiases. The purpose of this study was to investigate the influence of the nature of the cat tapeworm cysticerci exometabolites on some parameters of the proliferative activity of L929 fibroblast line. Fibroblasts were cultured on media containing cysticercal metabolites. The level of proliferative activity of fibroblasts was assessed by the number of mitoses and cloning efficiency. It was shown that exometabolites of feline cysticerci have wide spectra of biological action. The variety of effects caused by exometabolites probably allows the parasite to flexibly adapt to the conditions of parasitization in host tissues, act on various links of its homeostasis and maintain a certain level of specialization and functional activity of various target cells. Exometabolites of feline chain cysticerci alter the level of proliferative activity, the degree and direction of differentiation of connective tissue and epithelial cells in cultures. The nature of this effect is determined by the belonging of cells to one or another tissue type, the degree of their differentiation, as well as the dose of exometabolites and, probably, the ratio of their various components. The dependence of the nature of the influence of exometabolites of cysticerci on the proliferation of L929 cells on the presence of serum in the culture medium was studied. The cultivation of L929 fibroblasts on the medium with the greatest inhibitory effect was accompanied by a characteristic fatty degeneration of these cells, which can be considered as stimulation of differentiation of L929 fibroblasts along the pathway of conversion into adipocytes.Parasitosestissue helminthiasescysticerciproliferationfibroblastscell cultivationline L929cloning efficiencymitosisпаразитозытканевые гельминтозыцистицеркипролиферацияфибробластыкультивирование клетоклиния L929эффективность клонированиямитоз[1. Адоева, Е.Я. Использование различных экспериментальных моделей в изучении патогенеза и лечения ларвальных гельминтозов / Е.Я. Адоева, С.С. Козлов, В.И. Пустовойт // Вестник Российской Военно-медицинской академии. - 2018. - Т.64, №4. - С.6-9;][2. Березанцев, Ю.А. Проблема тканевого паразитизма / Ю.А. Березанцев // Паразитология. - 1982. - T.16, №4. - С.268-272;][3. Фадеев, Ф.А. Влияние состава ростовой среды и концентрации фетальной сыворотки на пролиферативную активность фибробластов дермы / Ф.А. Фадеев [и др.] // Гены и клетки. - 2016. - Т.11, №4. - С.75-79;][4. Березанцев, Ю.А. Инкапсуляция личинок паразитических нематод и цестод в тканях позвоночных как форма взаимоотношения паразита и хозяина / Ю.А. Березанцев, Д.В. Борщуков, И.В. Оксов, М.В. Чеснокова // Сборник трудов Зоологического института АН СССР (паразитология). - 1989. - №36. - С.131-160;][5. Калмыкова, Н.В. Биопластический материала на основе гиалуроновой кислоты как матрица для создания биомедицинских клеточных экспресс-продуктов для восстановления кожи / Н.В. Калмыкова, О.Г. Спичкина, В.Н. Эллиниди [и др.] // Гены и клетки. - 2014. - Т.9, №2. - С.68-75;][6. Ahearne, M. Combined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cells / М. Ahearne, J. Lysaght, A. Lynch // Cell Regeneration. - 2014. - №3. - P.13;][7. Liu, M. The effect of serum concentration on the growth, proliferation and collagen secretion in mouse L929 fibroblasts / M. Liu, P. Hu., K. Ding // Chinese J. Cell Mol. Immun. - 2011. - Vol.27, №7. - P.36-39;][8. Thangapazham, R. Alteration of skin properties with autologous dermal fibroblasts / R. Thangapazham, T. Darling, J. Meyerle // Int. J. Mol. Sci. - 2014. - Vol.15, №5. - P.8407-8427.]