Russian Military Medical Academy ReportsRussian Military Medical Academy Reports2713-23152713-2323Eco-Vector4342610.17816/rmmar43426Research ArticleANTI-FUNGAL ACTIVITY OF ACTINOMYCETES ALLOCATED FROM THE SURFACE OF ROOTS OF MEDICINAL PLANTSKhazievA. A-RyabovaO. B-KazakovaE. A-S.M. Kirov Military Medical Academy of the Ministry of Defense of the Russian FederationPerm State Pharmaceutical Academy of the Ministry of Health of the Russian Federation151220202S118118628082020Copyright © 2020, Khaziev A.A., Ryabova O.B., Kazakova E.A.2020The need for the use of antifungal drugs has increased significantly due to an increase in the prevalence of mycoses, due to the increased number of patients with immunosuppression. The introduction of new antifungal drugs, and, therefore, a preliminary study of the topic, expands the possibilities of treating fungal infections. Actinomycetes are the producers of most antimicrobials, among the drugs available on the market. Objective: to determine the antagonistic relationship of actinomycetes isolated from the surface of the roots of medicinal plants with pathogenic fungi of the mycelial and non-mycelial structure. The tasks were set: 1) to study the antagonistic activity of actinomycetes in relation to pathogenic fungi; 2) to identify active strains of actinomycetes for further study of the composition and mechanisms of action of their metabolites; 3) determine the best method for studying antagonistic activity; 4) to consider the stability of biologically active substances synthesized by actinomycetes in the presence of positive antagonistic relationships. The study of antifungal activity against yeast fungi was carried out by 2 methods: “perpendicular lines” and “blocks”, in order to identify the best. In a parallel study using the “block” method, we studied the duration of the activity of actinomycete metabolites, for which we measured the diameter of the growth suppression zone around the blocks for 1 week. The activity of actinomycetes against mycelial fungi was determined by the block method. All studies were performed in duplicate. Results: 1) of the 16 studied strains of actinomycetes, antifungal activity in at least one test culture was detected in 9 isolates, which is about 56%; 2) the best method of study was the "block" method, which allowed to identify 50% of the antagonists among the studied strains, while the "line" method, which is only 19%; 3) the most active actinomycromycetic isolate was the strain Streptomyces sp. with an index of 3-PP.ActinomycetalesCandidaAspergillusActinomycetalesCandidaAspergillusmycosiscandidiasisantagonistic activitypathogenic fungiradiant mushrooms“block” method“line” methodмикозкандидозантагонистическая активностьпатогенные грибылучистые грибыметод «блочков»метод «штриха»[1. Быков, А.С. Медицинская микробиология, вирусология и иммунология / А.С. Быков, В.В. Зверев. - М.: Изд-во МИА, 2018. - 336 с.][2. Гукова, Е.С. Проблемы медицинской микологии / Е.С. Гукова // Сборник работ Всероссийского конгресса по медицинской микробиологии, клинической микологии и иммунологии (СПб., НИИ Медицинской микологии им. П.Н. Кашкина, 2017 г.). - СПб., 2017. - С.18-23.][3. Дягтерева, А.С. Численность и структура актиномицетных комплексов корней некоторых видов лекарственных травянистых растений. Биосистемы: организация, поведение, управление / А.С. Дягтерева, М.А. Демидова, О.В. Рябова // Тезисы докладов 72-й Всероссийской с международным участием школы-конференции молодых ученых. - Нижний Новгород, 2019. - 74 с.][4. Санитарные Правила РФ №1.3.2322-08 от 15.08. - 2014 г. 5. Сбойчаков, В.Б. Медицинская микробиология: Учебник для курсантов и студентов факультетов подготовки врачей / В.Б. Сбойчаков. - СПб.: ВМедА, 2017. - 448 с.][6. Лабинская, А.С. Частная медицинская микробиология с техникой микробиологических исследований: Учебное пособие / А.С. Лабинская, А.С. Ещина, Л.П. Блинкова. - СПб., Изд-во Лань, 2017. - 608 с.][7. Jose, T. Candida albican; probale role in ecology of Early Childhood Caraies / T. Jose // Microbiology. - 2015. - 96 с. 8. Zuza-Alves D.L. An Update on Candida tropicalis Based on Basic and Clinical Approaches / D.L. Zuza-Alves, G.M. Chaves, W.P. Silva-Rocha // Microbiology. - 2017. - №8. - Р.4-9.]