Differences in the intron-retaining fragment of the shortened isoform of the NXF1 protein in different representatives of Rodentia



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Abstract

AIM:

The aim of this study was to analyze the species specificity of the sequence of the C-terminal fragment of the sNXF1 protein translated from the intron.

METHODS:

The study was performed using in silico methods, using the sequences of the Nxf1 gene of thirty-three representatives of the order Rodentia as materials, obtained from publicly available databases and NCBI data. 

RESULTS:

The analysis revealed that the C-terminal fragments of the sNXF1 of some representatives of the Rodentia order are of increased length. It was found that the cause of these extended fragments in all cases was the deletion of cytosine nucleotide. In all identified cases, regardless of the systematic position of the species, the extended fragment contains a sequence of conserved 13 a.a.

 

CONCLUSION:

The obtained results indicate that the analyzed sequence fragment does not influence the conformation of the protein sNxf1 in general. However, since the fragment has a specific folding pattern, it might define a set of RNP-complex partners.

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About the authors

Dmitrii D. Bondaruk

Federal State Budgetary Educational Institution of Higher Education "Saint-Petersburg State University"

Author for correspondence.
Email: D.D.Bondaruk@yandex.ru
ORCID iD: 0000-0003-0265-5759
SPIN-code: 8162-8441

Applican, Department of Genetics and Biotechnology.

Russian Federation, 199034, Russia, St. Petersburg, University emb. 7/9

Elena V. Golubkova

Federal State Budgetary Educational Institution of Higher Education "Saint-Petersburg State University"

Email: e.golubkova@spbu.ru
ORCID iD: 0000-0002-9528-5760
SPIN-code: 7386-1230

Cand. Sci. (Biology)

Russian Federation, 199034, Russia, St. Petersburg, University emb. 7/9

Ludmila A. Mamon

Federal State Budgetary Educational Institution of Higher Education "Saint-Petersburg State University"

Email: lmamon46@mail.ru
ORCID iD: 0000-0001-5338-0703
SPIN-code: 7780-6907

Doctor of Sci. (Biology)

Russian Federation, 199034, Russia, St. Petersburg, University emb. 7/9

Daria I. Pelle

ITMO University, Saint Petersburg, Russia

Email: kaisa23@ya.ru
ORCID iD: 0009-0002-0025-6631
SPIN-code: 6211-7251

Bachelor

Russian Federation, 197101, Russia, St. Petersburg, Kronverksky Pr. 49, bldg. A.

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