Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector11474310.17816/ecogen114743Genetic basis of ecosystems evolutionГенетические основы эволюции экосистемResearch ArticleMetabolite profiling of leaves of three Epilobium speciesМетаболическое профилирование листьев трех видов кипреяhttps://orcid.org/0000-0002-5862-26766399-2016PuzanskiyRoman K.ПузанскийРоман Константинович
Russian Federation

Cand. Sci. (Biol.), Research Associate, Laboratory of Analytical Phytochemistry; Department of Plant Physiology and Biochemistry

канд. биол. наук, научн. сотр., лаборатория аналитической фитохимии; кафедра физиологии и биохимии растений

puzansky@yandex.ruhttps://orcid.org/0000-0002-4663-83984273-1520SmirnovPavel D.СмирновПавел Дмитриевич
Russian Federation

Assistant Professor, Department of Botany

ассистент, кафедра ботаники

p.d.smirnov@gmail.comVanisovSergey A.ВанисовСергей Алексеевич
Russian Federation

Student, Department of Plant Physiology and Biochemistry

студент, кафедра физиологии и биохимии растений

s.vanisov@mail.ruDubrovskiyMaksim D.ДубровскийМаксим Дмитриевич
Russian Federation

Student, Department of Plant Physiology and Biochemistry

студент, кафедра физиологии и биохимии растений

max.d10@mail.ruhttps://orcid.org/0000-0003-1778-28145637-5122ShavardaAlexey L.ШавардаАлексей Леонидович
Russian Federation

Cand. Sci. (Biol.), Head of Laboratory of Analytical Phytochemistry; Center for Molecular and Cell Technologies

канд. биол. наук, заведующий лабораторией аналитической фитохимии; ресурсный центр «Развитие молекулярных и клеточных технологий»

stachyopsis@gmail.comhttps://orcid.org/0000-0003-3657-29867842-7611ShishovaMaria F.ШишоваМария Федоровна
Russian Federation

Dr. Sci. (Biol.), Professor, Department of Plant Physiology and Biochemistry

д-р биол. наук, профессор, кафедра физиологии и биохимии растений

mshishova@mail.ruhttps://orcid.org/0000-0003-2323-52359460-1278YemelyanovVladislav V.ЕмельяновВладислав Владимирович
Russian Federation

Cand. Sci. (Biol.), Associate Professor, Department of Genetics and Biotechnology

канд. биол. наук, доцент, кафедра генетики и биотехнологии

bootika@mail.ruSaint Petersburg State UniversityСанкт-Петербургский государственный университетKomarov Botanical Institute of the Russian Academy of SciencesБотанический институт им. В.Л. Комарова РАН28112022241220222042792932011202228112022Copyright ©; 2022, Puzanskiy R.K., Smirnov P.D., Vanisov S.A., Dubrovskiy M.D., Shavarda A.L., Shishova M.F., Yemelyanov V.V.Copyright ©; 2022, Пузанский Р.К., Смирнов П.Д., Ванисов С.А., Дубровский М.Д., Шаварда А.Л., Шишова М.Ф., Емельянов В.В.2022Puzanskiy R.K., Smirnov P.D., Vanisov S.A., Dubrovskiy M.D., Shavarda A.L., Shishova M.F., Yemelyanov V.V.Пузанский Р.К., Смирнов П.Д., Ванисов С.А., Дубровский М.Д., Шаварда А.Л., Шишова М.Ф., Емельянов В.В.https://creativecommons.org/licenses/by-nc-nd/4.0/https://journals.eco-vector.com/ecolgenet/article/view/114743

BACKGROUND: The ability of plants to adapt to oxygen deficiency is associated with the presence of various adaptations, many of which are mediated by significant changes of metabolism. These changes allow resistant wetland plants to grow even in oxygen-deficient environment.

AIM: The aim of the study was to carry out metabolic profiling of the leaves of the wetland species Epilobium palustre and Epilobium hirsutum, and the mesophyte species Epilobium angustifolium in order to identify the most characteristic metabolome traits of hypoxia-resistant plants.

MATERIALS AND METHODS: Metabolite profiling was performed by GC-MS. Statistical analysis of metabolomics data was processed using R 4.2.1 Funny-Looking Kid.

RESULTS: The resulting profile included about 360 compounds. 70 of these were identified and 50 compounds were determined to a class. Sugars (64) were the most widely represented in the obtained profiles. 16 amino and 20 carboxylic acids, lipids and secondary compounds have been identified. Significant differences were revealed between the profiles of leaf metabolomes of mesophyte E. angustifolium and hydrophytes E. hirsutum and E. palustre. The mesophyte was characterized by high levels of sugars. The metabolomes of wetland Epilobium species practically did not differ from each other and were characterized by the accumulation of amino acids, including GABA shunt intermediates, dicarboxylic acids of the Krebs cycle, and metabolites of glycolysis and lactic acid fermentation, which reflects the stimulation of anaerobic respiration, nitrogen metabolism, and alternative pathways of NAD(P)H reoxidation in wetland plants.

CONCLUSIONS: Traits of metabolic profiles detected in hydrophyte Epilobium species can be used to assess the degree of plant resistance to oxygen deficiency.

Способность растений адаптироваться к кислородной недостаточности связана с наличием различных приспособлений, многие из которых опосредованы существенными изменениями обмена веществ. Эти изменения позволяют устойчивым растениям-гидрофитам расти даже в дефицитной по кислороду среде. Цель настоящей работы состояла в метаболическом профилировании листьев гидрофитных видов Epilobium palustre, Epilobium hirsutum и мезофитного вида Epilobium angustifolium для выявления наиболее характерных изменений метаболома, свойственных устойчивым к дефициту кислорода растениям. Профилирование метаболитов проводили методом газовой хроматографии-масс-спектрометрии. Полученный профиль включал около 360 соединений. Из них было идентифицировано 70 и еще 50 соединений были определены до класса. В полученных профилях наиболее широко были представлены сахара (64) и их производные. Идентифицировано 16 аминокислот, 20 карбоновых кислот, а также липиды и вторичные соединения. Были выявлены существенные различия между профилями метаболомов листьев мезофитного E. angustifolium и гидрофитных E. hirsutum и E. palustre. Для мезофита были свойственны более высокие уровни сахаров. Метаболомы гидрофитных кипреев практически не отличались друг от друга и характеризовались аккумуляцией аминокислот, в том числе интермедиатов ГАМК-шунта, дикарбоновых кислот цикла Кребса и метаболитов гликолиза и молочнокислого брожения, что отражает стимуляцию у них анаэробного дыхания, азотного обмена и альтернативных путей реокисления НАД(Ф)Н.

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