Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector11741210.17816/ecogen117412Genetic basis of ecosystems evolutionГенетические основы эволюции экосистемResearch ArticleInositol phosphates’ synthesis in pea Pisum sativum L. root seedlings at the early stages after Rhizobium leguminosarum bv. viciae inoculationСинтез инозитолфосфатов в корнях проростков гороха Pisum sativum L. на ранних этапах после инокуляции Rhizobium leguminosarum bv. viciaehttps://orcid.org/0000-0003-4061-435X8119-0360BovinAndrey D.БовинАндрей Дмитриевич
Russian Federation

junior research associate

мл. научн. сотр, лаборатория сигнальной регуляции

andy-piter2007@mail.ruShirobokovaSvetlana A.ШиробоковаСветлана Алексеевна
Russian Federation

research engineer

инженер-исследователь, лаборатория сигнальной регуляции

schirobokova.s@gmail.comKarakashevGeorgy V.КаракашевГеоргий Васильевич
Russian Federation

Dr. Sci. (Biol.), head of the Laboratory of Analytical Toxicology

д-р биол. наук, заведующий лабораторией аналитической токсикологии

karakashev58@mail.ruhttps://orcid.org/0000-0002-5375-09434453-2060DolgikhElena A.ДолгихЕлена Анатольевна
Russian Federation

Dr. Sci. (Biol.), head of the Laboratory of Signal Regulation

д-р биол. наук, заведующая лабораторией сигнальной регуляции

dol2helen@yahoo.comAll-Russia Research Institute for Agricultural MicrobiologyВсероссийский научно-исследовательский институт сельскохозяйственной микробиологииResearch institute of hygiene, occupational pathology and human ecology, Federal medical biological agencyНаучно-исследовательский институт гигиены, профпатологии и экологии человека08032023120520232115171212202208032023Copyright ©; 2023, Eco-VectorCopyright ©; 2023, ООО "Эко-Вектор"2023Eco-VectorООО "Эко-Вектор"https://journals.eco-vector.com/ecolgenet/article/view/117412

Studying the role of inositol phosphates in the regulation of signal exchange between leguminous plants and nodule bacteria is of great interest since it affects the regulation of calcium level in the root cells in response to bacterial signals during symbiosis development. The regulation of intracellular calcium content is one of the key events in the control of symbiosis development, but remains very poorly understood. In present work, we revealed a significant increase in the content of inositol hexasphosphate (IP6), which occurs in response to the recognition of Nod factors and indicates that in plants, unlike animals, this form (along with the inositol triphosphate (IP3)) may be important for signal transduction. This is consistent with the data that receptor for IP3 in plants has not yet been found, despite numerous efforts.

Expression analysis of the genes encoding enzymes of two biosynthetic pathways for inositol phosphates showed stimulation of the PsITPK1 gene (Psat6g210960), which can control the phospholipid-independent pathway for synthesis of these compounds. Despite the fact that PsPIP5K (Psat5g134320) important for another pathway did not show increased expression in our experiments upon inoculation, the activation of the phospholipid-dependent pathway of inositol phosphate biosynthesis can be evidenced by stimulation of a number of genes encoding pospholipases C (PLCs) which were previously found in pea Pisum sativum as well as during analysis of transcriptome of Medicago truncatula root treated with Nod factors. Therefore, in plants, in contrast to animals, the pathways for the synthesis of inositol phosphates can be more diverse, which indicates the plasticity of signal pathways.

Изучение участия инозитолфосфатов в регуляции сигнального обмена между бобовыми растениями и клубеньковыми бактериями, представляет большой интерес, поскольку именно эти соединения могут влиять на изменение уровня кальция в клетках корня под влиянием сигналов бактерий при формировании симбиоза. Регуляция внутриклеточного содержания кальция является одним из ключевых событий в контроле развития симбиоза, но остается очень малоизученной. В данной работе мы выявили существенное увеличение содержания инозитолгексафосфата (ИФ6), происходящее в ответ на узнавание Nod-факторов и свидетельствующее о том, что у растений, в отличие от животных, эта форма (наряду с инозитолтрифосфатом (ИФ3)) может быть важна для передачи сигнала. Это соответствует данным о том, что у растений до сих пор не был обнаружен рецептор к ИФ3, несмотря на многочисленные попытки.

Анализ экспрессии генов, кодирующих ферменты двух путей биосинтеза инозитолфосфатов, показал стимуляцию гена PsITPK1 (Psat6g210960), который может контролировать фосфолипид-независимый путь синтеза этих соединений. Несмотря на то, что представитель другого пути PsPIP5K (Psat5g134320) не показал повышенной экспрессии в наших экспериментах при инокуляции, об активации фосфолипид-зависимого пути биосинтеза инозитолфосфатов может свидетельствовать стимуляция регуляторов этого пути, ряда генов фосфолипаз С (PLC), выявленная нами ранее у гороха Pisum sativum, а также при анализе транскриптомов корней Medicago truncatula, обработанных Nod-факторами. Таким образом, у растений в отличие от животных пути синтеза инозитолфосфатов могут быть более разнообразными, что свидетельствует о пластичности сигнальных путей.

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