Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector1253210.17816/ecogen1745-14Genetic basis of ecosystems evolutionГенетические основы эволюции экосистемResearch ArticleOrganization of the endoplasmic reticulum in cells of effective and ineffective pea nodules (Pisum sativum L.)Организация эндоплазматического ретикулума в клетках эффективных и неэффективных клубеньков гороха (Pisum sativum L.)https://orcid.org/0000-0003-3505-42989149-5662TsyganovaAnna V.ЦыгановаАнна Викторовна
Russian Federation

Candidate of Biological Sciences, Leading Scientist of the Laboratory of Molecular and Cellular Biology

Кандидат биологических наук, ведущий научный сотрудник лаборатории молекулярной и клеточной биологии

isaakij@mail.ruhttp://arriam.ru/departments/laboratoriya-molekulyarnoj-i-kletochnoj-biologii/https://orcid.org/0000-0003-3105-86896532-1332TsyganovViktor E.ЦыгановВиктор Евгеньевич
Russian Federation

Doctor of Biological Sciences, Head of the Laboratory of Molecular and Cellular Biology, Department of Biotechnology; Senior Scientist of Saint Petersburg Scientific Center RAS

Доктор биологических наук, заведующий лабораторией молекулярной и клеточной биологии; старший научный сотрудник Санкт-Петербургского научного центра РАН

tsyganov@arriam.spb.ruhttp://arriam.ru/departments/laboratoriya-molekulyarnoj-i-kletochnoj-biologii/All-Russia Research Institute for Agricultural MicrobiologyФГБНУ «Всероссийский научно-исследовательский институт сельскохозяйственной микробиологии»Saint Petersburg Scientific Center RASСанкт-Петербургский научный центр РАН14092019151220191745140405201914092019Copyright ©; 2019, Tsyganova A., Tsyganov V.Copyright ©; 2019, Цыганова А.В., Цыганов В.Е.2019Tsyganova A., Tsyganov V.Цыганова А.В., Цыганов В.Е.http://creativecommons.org/licenses/by/4.0https://journals.eco-vector.com/ecolgenet/article/view/12532

Background. The endoplasmic reticulum (ER) is the largest membrane-bound organelle, which plays an important role in the functioning of a plant cell and participates in its differentiation.

Materials and methods. Using the methods of transmission electron microscopy, the morphological features and dynamics of structural changes in the ER in symbiotic nodules of pea (Pisum sativum L.) wild-type and mutants blocked at different stages of nodule development were studied.

Results. ER developed from a network of individual tubules in meristematic cells, to a developed network of cisterns around the nucleus and plasmalemma, and a network of granular and smooth tubules accompanying infection structures in colonized and infected cells and symbiosomes in infected cells.

Conclusions. A correlation was found between the level of development of the ER network and the degree of bacteroid differentiation.

Эндоплазматический ретикулум (ЭПР) является самой большой, окруженной мембраной органеллой, которая выполняет важную роль в функционировании растительной клетки и участвует в ее дифференцировке. С помощью методов просвечивающей электронной микроскопии были исследованы морфологические особенности и динамика структурных изменений ЭПР в симбиотических клубеньках гороха (Pisum sativum L.) дикого типа и мутантов, блокированных на различных стадиях развития клубенька. ЭПР развивался от сети отдельных канальцев в меристематических клетках, к развитой сети цистерн вокруг ядра и плазмалеммы и сети гранулярных и гладких канальцев, сопровождающих инфекционные структуры в колонизированных и инфицированных клетках и симбиосомы в инфицированных клетках. Была выявлена корреляция между уровнем развития сети ЭПР и степенью дифференцировки бактероидов.

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