Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector248110.17816/ecogen13277-99ArticlesСтатьиResearch ArticleTransgenic plants as bioreactors for the production of substances of medicinal and veterinary importanceТрансгенные растения-продуценты веществ медицинского и ветеринарного назначенияSavelevaNatalia VladimirovnaСавельеваНаталья Владимировна

к. б. н., доцент

nata.saveljeva@gmail.comBurlakovskiyMikhail SergeevichБурлаковскийМихаил Сергеевич

Dept. Of Genetics and Biotechnology

аспирант, кафедра генетики и биотехнологии

burmish@yandex.ruYemelyanovVladislav VladimirovichЕмельяновВладислав Владимирович

Dept. Of Genetics and Biotechnology

к. б. н., доцент, кафедра генетики и биотехнологии

bootika@mail.ruLutovaLyudmila AlekseevnaЛутоваЛюдмила Алексеевна

Dept. Of Genetics and Biotechnology

д. б. н., профессор, кафедра генетики и биотехнологии

la.lutova@gmail.comLab. UMRSt. Petersburg State UniversityСПбГУ15062015132VOL 13, NO2 (2015)ТОМ 13, №2 (2015)779930032016Copyright ©; 2015, Saveleva N.V., Burlakovskiy M.S., Yemelyanov V.V., Lutova L.A.Copyright ©; 2015, Савельева Н.В., Бурлаковский М.С., Емельянов В.В., Лутова Л.А.2015Saveleva N.V., Burlakovskiy M.S., Yemelyanov V.V., Lutova L.A.Савельева Н.В., Бурлаковский М.С., Емельянов В.В., Лутова Л.А.http://creativecommons.org/licenses/by/4.0https://journals.eco-vector.com/ecolgenet/article/view/2481

The use of plants as bioreactors has become of a great importance in the modern biotechnology. The transgenic plants are capable of synthesizing of many substances, including valuable pharmaceuticals. Plants possess a number of advantages compared to conventional bioreactors - microorganisms and animal cell cultures. The product safety and lower production costs are among them. One of the promising directions in plant biotechnology is the creation of “edible vaccines, plantibodies and adjuvants” based on recombinant antigens, immunoglobulins and immunoregulatory cytokines. Edible bioreactor plants can be administered as food and feed additives in medicine and veterinary avoiding expensive purification procedures. Interferons have antiviral, antibacterial, antitumor and immunomodulatory activity, and are implicated in the prophylaxis and therapy of diseases of different etiologies. Investigations concerning with obtaining of bioreactor plants synthesizing γ-interferons of mammals and birds are carried out in the laboratory of genetic and cellular engineering of plants St. Petersburg State University. Our recent achievements in the creation of inbreed tobacco line producing bovine γ-interferon are described.

Использование растительных систем в качестве биореакторов приобретает все большее значение в современной биотехнологии. С помощью трансгенных растений получают множество веществ, в том числе используемых в фармацевтике. Преимуществом растений являются безопасность продукта и меньшие затраты на его производство по сравнению с традиционными биопродуцентами - микроорганизмами и клеточными культурами. Одним из перспективных направлений в получении растений-продуцентов является создание «съедобных вакцин и адъювантов» на основе рекомбинантных антигенов и иммунорегуляторных цитокинов, которые планируется использовать в качестве биологических добавок в животноводстве. В лаборатории генной и клеточной инженерии растений СПбГУ проводятся исследования по получению растений-продуцентов, синтезирующих γ-интерфероны млекопитающих и птиц, обладающих антивирусной, антимикробной, противоопухолевой и иммуномодулирующей активностью. В статье рассмотрены основные этапы создания стабильной генетической линии, как продуцента фармакологического белка.

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