Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector248410.17816/ecogen132127-135ArticlesСтатьиResearch ArticleObtaining of transgenic alfalfa for improved phytoremediation the petroleum contaminated soilsПолучение трансгенных растений люцерны посевной (Medicago Sativa L.) для повышения эффективности фиторемедиации нефтезагрязненных почвStepanovaAnna YurievnaСтепановаАнна Юрьевна

senior researcher, PhD, group of specialized metabolism of roots

с. н. с., к. б. н., группа специализированного метаболизма корней

step_ann@mail.ruOrlovaEkaterina VladimirovnaОрловаЕкатерина Владимировна

researcher, group of specialized metabolism of roots

н. с., группа специализированного метаболизма корней

ekatia@inbox.ruTereshonokDmitriy ViktorovichТерешонокДмитрий Викторович

researcher, PhD, laboratory of genetics of cell cultures

н. с., к. б. н., лаборатория генетики культивируемых клеток

diman_ter_vi@mail.ruDolgikhYulia IvanovnaДолгихЮлия Ивановна

head of the lab, prof., MD, group of specialized metabolism of roots

д. б. н., зав. лаб, профессор, лаборатория генетики культивируемых клеток

ivan-d1@yandex.ruTimiryazev institute of plant physiology, RASИФР РАН15062015132VOL 13, NO2 (2015)ТОМ 13, №2 (2015)12713530032016Copyright ©; 2015, Stepanova A.Y., Orlova E.V., Tereshonok D.V., Dolgikh Y.I.Copyright ©; 2015, Степанова А.Ю., Орлова Е.В., Терешонок Д.В., Долгих Ю.И.2015Stepanova A.Y., Orlova E.V., Tereshonok D.V., Dolgikh Y.I.Степанова А.Ю., Орлова Е.В., Терешонок Д.В., Долгих Ю.И.http://creativecommons.org/licenses/by/4.0https://journals.eco-vector.com/ecolgenet/article/view/2484

Background. The possibility of using transgenic plants and their complexes with microorganisms to clean up soil from oil pollution is a topical area of researches. In our work the transgenic alfalfa plants with a gene rhlA, responsible for the biosynthesis of biosurfactant - ramnolipid, were obtained. Rhamnolipids help to reduce the surface tension of the hydrocarbon oil and its desorbtion from soil particles, thereby facilitating its recycling by microorganism. Material and methods. The protocol for agrobacterium-mediated transformation was optimized, transgenic alfalfa plants with a gene rhlA were obtained and their status was confirmed by molecular analysis. Results. Cultivation of the control and the transgenic alfalfa plants in soil polluted with 4 % oil showed the advantage of plants emitting ramnolipids: recycle oil was 71 % for 56 days and it was 20 % higher compared with the control plants. When used together, the transgenic plants and microorganism Candida maltosa increased the degree of degradation of the oil to 86 %. Conclusion. The results suggest promising application of transgenic plants and the complex “transgenic plants - microorganisms” to increase the efficiency of bioremediation.

Исследована возможность использования трансгенных растений и их комплекса с микроорганизмами для очистки почвы от нефтезагрязнений. Подобраны условия агробактериальной трансформации и получены трансгенные растения люцерны с геном rhlA, ответственным за биосинтез биосурфактанта - рамнолипида. Выращивание в почве, содержащей 4 % нефти, контрольных и трансгенных растений люцерны показало преимущество растений, выделяющих рамнолипиды: утилизация нефти была на 20 % выше по сравнению с контролем. При совместном использовании трансгенных растений и микроорганизмов Candida maltosа степень утилизации нефти удалось повысить до 86 %. Полученные результаты свидетельствуют о перспективности применения трансгенных растений и их комплекса с микроорганизмами для повышения эффективности биоремедиации.

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