Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector4864610.17816/ecogen48646Genetic basis of ecosystems evolutionГенетические основы эволюции экосистемResearch ArticleCreation of an inducible vector system based on the rhizobia nodA gene promoterСоздание индуцируемой векторной системы на основе промотора гена nodA ризобийhttps://orcid.org/0000-0001-7553-9527A-3397-20143297-4830ChubukovaOlga V.ЧубуковаОльга Вячеславовна
Russian Federation

PhD, Cand. Sci. (Biol.), Researcher

канд. биол. наук, научный сотрудник

chubukova@bk.ruhttps://orcid.org/0000-0001-5480-53201866-7896VershininaZilya R.ВершининаЗиля Рифовна
Russian Federation

PhD, Cand. Sci. (Biol.), Researcher

канд. биол. наук, научный сотрудник

zilyaver@mail.ru6798-7913MatnyazovRustam T.МатниязовРустам Тахирович
Russian Federation

PhD, Cand. Sci. (Biol.)

канд. биол. наук, научный сотрудник

rmat@mail.ruhttps://orcid.org/0000-0001-6637-93651919-5236BaymievAndrey K.БаймиевАндрей Ханифович
Russian Federation

PhD, Cand. Sci. (Biol.)

д-р биол. наук, ведущий научный сотрудник

baymiev@anrb.ruhttps://orcid.org/0000-0003-0606-67403771-4063BaymievAleksey K.БаймиевАлексей Ханифович
Russian Federation

PhD, Cand. Sci. (Biol.), Head of Laboratory

д-р биол. наук, заведующий лабораторией

alex@anrb.ruInstitute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of SciencesИнститут биохимии и генетики — обособленное структурное подразделение Федерального государственного бюджетного научного учреждения Уфимского федерального исследовательского центра Российской академии наук250220211503202119113212810202025022021Copyright ©; 2021, Chubukova O.V., Vershinina Z.R., Matnyazov R.T., Baymiev A.K., Baymiev A.К.Copyright ©; 2021, ООО "Эко-Вектор"2021Chubukova O.V., Vershinina Z.R., Matnyazov R.T., Baymiev A.K., Baymiev A.К.ООО "Эко-Вектор"https://journals.eco-vector.com/ecolgenet/article/view/48646

BACKGROUND: The possibility of changing the properties of rhizobial bacteria by giving them the ability to regulate the expression of additionally introduced genes into them is an urgent task both for fundamental science and for applied agrobiology, since this will make it possible to obtain microsymbionts with desired properties. An expression construct using the rhizobia regulatory system was created in this work. The rhizobia nodD gene encodes a regulatory protein that, in the presence of plant inducers, flavonoids, activates the transcription of nod-genes involved in the early stages of the formation of legume-rhizobium symbiosis.

MATERIALS AND METHODS: A vector construct containing the nodD gene from Rhizobium leguminosarum bv. trifoli under the regulation of its own promoter and the gfp gene under the regulation of the nodA gene promoter from the same rhizobia was obtained. Neorhizobium galegae CIAM 0702 were transformed with the vector construct.

RESULTS: It has been shown that in recombinant strains synthetic flavonoids are capable of inducing expression of gfp gene to varying degrees.

CONCLUSION: In the future, the results can be used to obtain rhizosphere microorganisms with a controlled synthesis of growth-stimulating and protective substances.

Введение. Возможность изменения свойств ризобиальных бактерий путем придания им способности регулировать экспрессию привнесенных в них дополнительно генов является актуальной задачей как для фундаментальной науки, так и для прикладной агробиологии, поскольку это позволит создавать микросимбионтов с заданными свойствами. Нами создана экспрессионная конструкция с применением регуляторной системы самих ризобий. Ген nodD ризобий кодирует регуляторный белок, который в присутствии растительных индукторов, флавоноидов, активирует транскрипцию nod-генов, участвующих в ранних этапах формирования бобово-ризобиального симбиоза.

Материалы и методы. Был получен вектор, содержащий ген nodD из Rhizobium leguminosarum bv. trifoli под регуляцией собственного промотора и ген gfp под регуляцией промотора гена nodA из того же организма.

Результаты. Показано, что в рекомбинантных штаммах ризобий Neorhizobium galegae CIAM 0702 синтетические флавоноиды способны в разной степени индуцировать экспрессию гена зеленого флуоресцентного белка GFP.

Выводы. В дальнейшем полученные результаты могут быть использованы для получения ризобиальных микроорганизмов с регулируемым синтезом ростостимулирующих и защитных веществ.

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