Ecological geneticsEcological genetics1811-09322411-9202Eco-Vector4864610.17816/ecogen48646Research ArticleCreation of an inducible vector system based on the rhizobia nodA gene promoterChubukovaOlga V.<p>PhD, Cand. Sci. (Biol.), Researcher</p>chubukova@bk.ruhttps://orcid.org/0000-0001-7553-9527VershininaZilya R.<p>PhD, Cand. Sci. (Biol.), Researcher</p>zilyaver@mail.ruhttps://orcid.org/0000-0001-5480-5320MatnyazovRustam T.<p>PhD, Cand. Sci. (Biol.)</p>rmat@mail.ruBaymievAndrey K.<p>PhD, Cand. Sci. (Biol.)</p>baymiev@anrb.ruhttps://orcid.org/0000-0001-6637-9365BaymievAleksey K.<p>PhD, Cand. Sci. (Biol.), Head of Laboratory</p>alex@anrb.ruhttps://orcid.org/0000-0003-0606-6740Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences1503202119113212810202025022021Copyright © 2021, Chubukova O.V., Vershinina Z.R., Matnyazov R.T., Baymiev A.K., Baymiev A.К.2021<p><em>BACKGROUND</em><em>:</em> The possibility of changing the properties of rhizobial bacteria by giving them the ability to regulate the expression of additionally introduced genes into them is an urgent task both for fundamental science and for applied agrobiology, since this will make it possible to obtain microsymbionts with desired properties. An expression construct using the rhizobia regulatory system was created in this work. The rhizobia <em>nodD</em> gene encodes a regulatory protein that, in the presence of plant inducers, flavonoids, activates the transcription of nod-genes involved in the early stages of the formation of legume-rhizobium symbiosis.</p>
<p><em>MATERIALS AND METHODS: </em>A vector construct containing the <em>nodD</em> gene from <em>Rhizobium leguminosarum</em> bv. <em>trifoli</em> under the regulation of its own promoter and the <em>gfp</em> gene under the regulation of the <em>nodA</em> gene promoter from the same rhizobia was obtained. N<em>eorhizobium galegae</em> CIAM 0702 were transformed with the vector construct.</p>
<p><em>RESULTS:</em> It has been shown that in recombinant strains synthetic flavonoids are capable of inducing expression of <em>gfp</em> gene to varying degrees.</p>
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