Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector5377110.17816/ecogen53771Genetic basis of ecosystems evolutionГенетические основы эволюции экосистемResearch ArticleAnalysis of the expression of polyamine biosynthesis genes in nodules of the garden pea (Pisum sativum L.) and the effect of exogenous treatment with polyamines on their developmentАнализ экспрессии генов синтеза полиаминов в клубеньках гороха посевного (Pisum sativum L.) и влияние экзогенной обработки полиаминами на их развитиеhttps://orcid.org/0000-0002-9119-065X1104-7503IvanovaKira A.ИвановаКира Андреевна
Russian Federation

junior researcher

мл. н. с.

kivanova@arriam.ruhttps://orcid.org/0000-0003-3105-86897006136325Q-5634-20166532-1332TsyganovViktor E.ЦыгановВиктор Евгеньевич
Russian Federation

Dr. Sci. (Biol.)

д-р биол. наук

vetsyganov@arriam.ruAll Russia Research Institute for Agricultural MicrobiologyВсероссийский научно-исследовательский институт сельскохозяйственной микробиологии10082021101020211931972080712202010082021Copyright ©; 2021,Copyright ©; 2021, ООО "Эко-Вектор"2021ООО "Эко-Вектор"https://journals.eco-vector.com/ecolgenet/article/view/53771

BACKGROUND: Polyamines are acting as signaling molecules during adaptation to stressful environment and as regulators of plant development. In plants, polyamines are represented mainly by putrescine, spermidine and spermine. The concentration of polyamines in symbiotic nodules of some legumes is 5–10 times higher than in the other organs, which indicates their important role in the formation and functioning of symbiotic nodules.

MATERIALS AND METHODS: We analyzed the expression of genes encoding polyamine biosynthesis enzymes in symbiotic nodules, as well as the effect of exogenous polyamines on the nodule number and the average nodule weight in wild-type SGE plants and symbiotic pea mutants SGEFix–-1 (sym40-1) and SGEFix–-2 (sym33-3).

RESULTS: The comparable expression level of arginine decarboxylase gene (PsADC) was observed in all analyzed nodules, whereas the expression level of ornithine decarboxylase gene (PsODC), was highly increased in nodules of SGEFix–-2 (sym33-3) mutant. Treatment of the root system with a 0.1 mM solution of polyamines mixture led to an increase in the average weight of the nodule in wild-type plants and in the SGEFix–-2 (sym33-3) mutant plants.

CONCLUSIONS: It was shown that the main pathway of putrescine synthesis in wild-type pea symbiotic nodules is the arginine pathway, while the ornithine pathway is probably associated with activation of plant defense reactions. Polyamines acting, apparently, through ethylene, affect the functioning of the nodule meristem.

Введение. Концентрация полиаминов в симбиотических клубеньках некоторых бобовых в 5–10 раз превышает их концентрацию в других органах, что указывает на их важную роль в формировании и функционировании симбиотических клубеньков.

Материалы и методы. В рамках данной работы был проведен анализ экспрессии генов, кодирующих ферменты биосинтеза полиаминов, в симбиотических клубеньках, а также изучено влияние экзогенных полиаминов на клубенькообразование у растений линии дикого типа SGE и симбиотических мутантов гороха SGEFix–-1 (sym40-1) и SGEFix–-2 (sym33-3).

Результаты. Было показано, что основной путь синтеза путресцина в симбиотических клубеньках растений дикого типа — аргининовый, тогда как у мутанта SGEFix–-2 (sym33-3) активируется также орнитиновый путь. Обработка корневой системы 0,1 мМ раствором смеси полиаминов приводила к увеличению среднего веса клубенька у растений дикого типа и мутанта SGEFix–-2 (sym33-3).

Заключение. Таким образом, полиамины, действуя, по-видимому, через этилен, влияют на функционирование меристемы клубеньков.

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