Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector62419710.17816/ecogen624197Genetic basis of ecosystems evolutionГенетические основы эволюции экосистемResearch ArticleGenetic polymorphism in waxweed (Red Book of the Russian Federation) in North-West RussiaГенетический полиморфизм восковника болотного (Красная книга Российской Федерации) на северо-западе Россииhttps://orcid.org/0000-0001-9923-86144663-2218TikhodeyevOleg N.ТиходеевОлег Николаевич
Russian Federation

Cand. Sci. (Biology), Associate Professor

канд. биол. наук, доцент

tikhodeyev@mail.ruhttps://orcid.org/0000-0002-9347-67582573-2107TikhodeevaMarina Y.ТиходееваМарина Юрьевна
Russian Federation

Cand. Sci. (Biology)

канд. биол. наук

marinaur@list.ruhttps://orcid.org/0009-0004-7839-84828364-4430RomanovichAnna E.РомановичАнна Эдуардовна
Russian Federation

Cand. Sci. (Biology)

канд. биол. наук

aromanovich@gmail.comGalaktionovaUliyana A.ГалактионоваУльяна Андреевна
Russian Federation
ugalaktionova@alkorbio.ruSemichevaOlga A.СемичеваОльга Андреевна
Russian Federation
osemicheva@alkorbio.ruhttps://orcid.org/0009-0007-5126-6035BolshakovVyacheslav N.БольшаковВячеслав Николаевич
Russian Federation
vbolshakov@alkorbio.ruSaint Petersburg State UniversityСанкт-Петербургский государственный университетVega Ltd, Alkor Bio GroupООО «Вега ГК Алкор Био»07122023170720242221251380412202307122023Copyright ©; 2024, Eco-VectorCopyright ©; 2024, Эко-Вектор2024Eco-VectorЭко-Векторhttps://journals.eco-vector.com/ecolgenet/article/view/624197

BACKGROUND: Waxweed (Myrica gale L.) is a protected plant species found in the Russian Federation only in the Leningrad Region and Karelia. It is almost not studied from the genetic point of view. This species is presumably hexaploid; it propagates mainly vegetatively, and the role of sexual reproduction in the life cycle of waxweed is unclear. All waxweed populations in the Russian Federation are small and belong to the edge ones (occupy the easternmost positions in the range).

AIM: The aim of the study is to investigate genomic DNA polymorphism in waxweed populations in the Russian Federation, and to evaluate the role of sexual reproduction in propagation and dispersal of this species.

MATERIALS AND METHODS: AFLP-analysis of waxweed genomic DNA in 6 natural populations (5 populations in the Leningrad Region, 1 in Karelia). Using 3 pairs of primers, we studied waxweed genomic polymorphism for 22 DNA fragments.

RESULTS: Each of the studied waxweed populations is genetically polymorphic. Among 182 analyzed plants we distinguished 27 different AFLP-genotypes, two of which were common in all populations studied. Most of others AFLP genotypes (20) were represented just by a single plant or a couple of plants. Some of these rare AFLP genotypes are likely the results of mutation and/or recombination processes affecting the common AFLP genotypes.

CONCLUSIONS: The role of sexual reproduction in waxweed propagation, although minor, is noticeable.

Актуальность. Восковник болотный (Myrica gale L.) — охраняемый вид растений, встречающийся в Российской Федерации только в Ленинградской области и в Карелии. С генетической точки зрения практически не изучен. Предположительно гексаплоиден, размножается главным образом вегетативно, роль полового размножения в воспроизведении восковника не ясна. Все популяции этого вида на территории Российской Федерации малочисленны и относятся к периферическим (занимают в ареале крайнее восточное положение).

Цель работы — исследовать полиморфизм геномной ДНК в популяциях восковника на территории Российской Федерации, оценить роль полового размножения в воспроизведении и расселении данного вида.

Материалы и методы. AFLP-анализ геномной ДНК восковника из 6 природных популяций (5 популяций в Ленинградской области, 1 в Карелии). Используя 3 пары праймеров, мы исследовали генетический полиморфизм восковника по 22 фрагментам ДНК.

Результаты. Каждая из исследованных популяций восковника генетически полиморфна. Среди 182 проанализированных растений мы выявили 27 AFLP-генотипов, два из которых обычны для всех 6 популяций. Большинство остальных AFLP-генотипов (20) обнаружены всего у одного или двух растений. Некоторые из этих редких AFLP-генотипов, по-видимому, являются результатами мутационных и/или рекомбинационных процессов на основе обычных AFLP-генотипов.

Заключение. Наличие потенциально рекомбинантных AFLP-генотипов позволяет предположить, что в воспроизведении восковника роль полового размножения хотя и минорна, но все же заметна.

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