Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector630310.17816/ecogen15157-63ArticlesСтатьиResearch ArticleInvestigation of paraoxonase 1 activity of the workers at the plant, who have long-term contact with organophosphorus compoundsИсследование активности параоксоназы 1 у работников предприятий, длительно контактирующих с фосфорорганическими соединениямиRazgildinaNatalia DРазгильдинаНаталья Дмитриевна
Russian Federation

Senior laboratory, Molecular and Radiation Biophysics Department

старший лаборант, лаборатория молекулярной генетики человека

razgnata@mail.ruMiroshnikovaValentina VМирошниковаВалентина Вадимовна
Russian Federation

Researcher, Molecular and Radiation Biophysics Department

научный сотрудник, лаборатория молекулярной генетики человека

mutantropol@mail.ruFomichevAleksey VФомичевАлексей Вячеславович
Russian Federation

Senior lecturer, Department of military field therapy

старший преподаватель, кафедра военно-полевой терапии

fomichoff74@mail.ruMalishevaEkaterina VМалышеваЕкатерина Викторовна
Russian Federation

Intern doctor, Department of military field therapy

врач-интерн, кафедра военно-полевой терапии

katerinamalisheva@mail.ruPanteleevaAlexandra AПантелееваАлександра Андреевна
Russian Federation

Trainee researcher, Molecular and Radiation Biophysics Department

стажер-исследователь, лаборатория молекулярной генетики человека

aleksandra9122@mail.ruPchelinaSofia NПчелинаСофья Николаевна
Russian Federation

Head of the laboratory, Molecular and Radiation Biophysics Department

заведующая лабораторией, лаборатория молекулярной генетики человека

sopchelina@hotmail.comB.P. Konstantinov Nuclear Physics Institute in Saint PetersburgФБГУ «Петербургский институт ядерной физики им. Б.П. Константинова»S.M. Kirov Military Medical AcademyФГБВОУ ВО «Военно-медицинская академия им. С.М. Кирова»15032017151VOL 15, NO1 (2017)ТОМ 15, №1 (2017)576310052017Copyright ©; 2017, Razgildina N.D., Miroshnikova V.V., Fomichev A.V., Malisheva E.V., Panteleeva A.A., Pchelina S.N.Copyright ©; 2017, Разгильдина Н.Д., Мирошникова В.В., Фомичев А.В., Малышева Е.В., Пантелеева А.А., Пчелина С.Н.2017Razgildina N.D., Miroshnikova V.V., Fomichev A.V., Malisheva E.V., Panteleeva A.A., Pchelina S.N.Разгильдина Н.Д., Мирошникова В.В., Фомичев А.В., Малышева Е.В., Пантелеева А.А., Пчелина С.Н.http://creativecommons.org/licenses/by/4.0https://journals.eco-vector.com/ecolgenet/article/view/6303

Background. Liver enzyme paraoxonase 1 (PON1) plays an important role in protection the organism from toxic effects of organophosphorus compounds (OPs) via their hydrolysis whose rate and efficiency depend on PON1 serum level activity. PON1 activity is largely determined by the polymorphic variants of the PON1 gene. Effect of long-term work with exposure to the toxic OPs on the PON1 activity is almost unknown. The aim of the present work was to study the effect of long-term work with exposure to the toxic OPs on PON1 serum enzymatic activity depending on polymorphisms Q191R, L54M, C(-108)T PON1 gene.

Materials and methods. PON1 serum enzymatic activity and PON1 polymorphisms were determined in men, who were categorized in 2 groups: workers of companies providing storage and disposal of the OPs (68) and control group (37). The PON1 191, PON1 55 and PON1 108 polymorphisms were studied by polymerase chain reaction/restriction fragment length polymorphism. PON1 serum enzymatic activity was measured by a spectrophotometric method using paraoxon.

Results. PON1 activity in workers with exposure to the toxic OPs relative was increased compared to the control group (p = 0,027). Differences in serum PON1 activity was shown for the carriers of certain genotypes of the PON1 gene: PON1 serum activity was higher in workers compared to controls only for LL genotype (L54M polymorphism) and C allele (C(-108)T polymorphism) carriers (p < 0,001 and p = 0,002, correspondently).

Conclusion. We suggest that the increase in serum PON1 activity in workers providing storage and disposal of OPs could be modulated with the polymorphic variants of the PON1 gene.

Фермент печени параоксоназа 1 (PON1) играет важную роль в защите организма от токсического действия фосфорорганических соединений (ФОС) посредством их гидролиза. Активность PON1 определяется полиморфными локусами гена PON1. В данной работе методом полимеразной цепной реакции c последующим рестрикционным анализом было произведено типирование полиморфных локусов Q191R, L54M, C(-108)T гена PON1, была измерена активность PON1 у 68 мужчин, работников предприятий, длительно контактирующих с ФОС, и в контрольной группе (N = 37). Активность PON1 у работников предприятий относительно субстрата параоксон была повышена по сравнению с контрольной группой (p < 0,05). Показано, что увеличение активности PON1 у работников предприятий, длительно контактирующих с ФОС, модулируется генотипами гена PON1.

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