Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector69579110.17816/ecogen695791Genetically modified organism.history, achievements, social and environmental risks.«ГМО: ИСТОРИЯ, ДОСТИЖЕНИЯ, СОЦИАЛЬНЫЕ И ЭКОЛОГИЧЕСКИЕ РИСКИ»Evaluation of selective agent requirements for pea callus culture expressing foreign DNAhttps://orcid.org/0000-0002-9037-4684SimonovaVeronika Y.СимоноваВероника Юрьевна
Russian Federation

student

nikasimonova14@gmail.comhttps://orcid.org/0000-0002-5045-2641PotsenkovskaiaElina A.
Russian Federation

specialist in plant biology and biotechnology

potsenkovskaya.ea@talantiuspeh.ruhttps://orcid.org/0009-0006-9753-2004VaninaAlexandra A.
Russian Federation
alexandraspb15@gmail.comKiselevaAnna S.
Russian Federation

student

anykisely@gmail.comhttps://orcid.org/0000-0002-9458-01949877-5352MatveenkoAndrew G.МатвеенкоАндрей Георгиевич
Russian Federation

Cand. Sci. (Biology)

кандидат биологических наук

a.matveenko@spbu.ruhttps://orcid.org/0009-0006-7828-4105PavlovaDaria B.
Russian Federation
db_pavlova@mail.ruhttps://orcid.org/0000-0002-2875-3798SmirnovKirill V.
Russian Federation
kirill.vad.smirnov@gmail.comhttps://orcid.org/0000-0002-2565-1155EfremovaElena P.
Russian Federation

student in biology, department of genetics and biotechnology

elena.efremova@spbu.ru8700-4413BrynchikovaAnna V
Russian Federation
annbv19@gmail.comhttps://orcid.org/0000-0002-0549-1457TvorogovaVarvara E.
Russian Federation

phd, senior researcher, department of genetics and biotechnology

krubaza@mail.ruSirius UniversitySt Petersburg State UniversityСанкт-Петербургский государственный университетSaint Petersburg State UniversityСанкт-Петербургский государственный университетSt Petersburg State UniversityСанкт-Петербургский Государственный УниверситетAll-Russia Research Institute for Agricultural MicrobiologyВсероссийский научно-исследовательский институт сельскохозяйственной микробиологииSaint Petersburg State UniversitySirius University of Science and TechnologyНаучно-технологический университет «Сириус»031220252410411202529112025Copyright ©; , Eco-VectorCopyright ©; , Эко-ВекторEco-VectorЭко-Векторhttps://eco-vector.com/for_authors.php#07https://journals.eco-vector.com/ecolgenet/article/view/695791

BACKGROUND: Genetic modification of pea remains challenging, which can likely be attributed to its low regeneration efficiency. To obtain transgenic pea plants, shoot regeneration followed by rooting is usually applied. Another regeneration pathway, somatic embryogenesis, is not used for pea genome modification due to very low frequency of this process. If a gene stimulating somatic embryogenesis was identified, it could be used as a morphogenic regulator to enable regeneration of pea plants from genetically modified callus cells. The search for such genes relies on the development of a cultivation system, allowing production of a significant amount of callus tissue in which a potential morphogenic regulator is ectopically expressed.AIM: The aim of study was to evaluate the possibility of obtaining pea callus tissue expressing foreign DNA with or without usage of selective agents, specifically, kanamycin and hygromycin B.MATERIALS AND METHODS: In this study, we combined agrobacterial transformation protocol with a method of callus induction from pea shoot apex explants. To evaluate the effectiveness of this transformation system with different selective agents, we used two different reporters: RUBY and DsRed.RESULTS AND CONCLUSIONS: Our results demonstrate that transformation of pea shoot apices using the developed system yields a significant percentage of calli containing tissue regions which express foreign DNA. Addition of the antibiotics as selective agents doesn’t increase frequency of calli expressing introduced DNA. Results obtained in this study suggest that searching for regeneration stimulators is feasible in Pisum sativum without usage of selective agents.

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