Ecological geneticsEcological geneticsЭкологическая генетика1811-09322411-9202Eco-Vector7075910.17816/ecogen70759Genetic toxicologyГенетическая токсикологияResearch ArticleModerate thermophilic chemoorganoheterotrophic bacterium in surface layer of anthropogenic grounds of industrial estate area of Al-Mafraq, JordanУмеренно термофильная хемоорганогетеротрофная бактерия из поверхностного слоя антропогенного грунта промышленной зоны г. Аль-Мафрак, Иорданияhttps://orcid.org/0000-0002-0387-79976603847300I-4980-20159759-9942GalushkoAlexander S.ГалушкоАлександр С.
Russian Federation

PhD, Cand. Sci. (Med.)

канд. биол. наук

galushkoas@inbox.ruhttps://orcid.org/0000-0001-8618-5795IbryaevaSnezanna K.ИбряеваСнежанна К.
Russian Federation

student

студентка

snezhanna.ibryaeva@yandex.ruhttps://orcid.org/0000-0001-7204-965357221754069I-8764-20183084-1394ZhuravlevaAnna S.ЖуравлеваАнна С.
Russian Federation

junior researcher, postgraduate student

мл. н. сотр., аспирант

zhuravlan@gmail.comhttps://orcid.org/0000-0002-1132-99157003272258AAN-6594-20209260-4501PanovaGayane G.ПановаГаяне Г.
Russian Federation

PhD, Cand. Sci. (Med.), major researcher

канд. биол. наук, гл. н. сотр.

gaiane@inbox.ruhttps://orcid.org/0000-0003-2410-822X36025359900JacobJacob H.ЯкобЯкоб Х.
Jordan

PhD, Professor

PhD, профессор

jjacob@aabu.edu.joAgrophysical Research InstituteАгрофизический научно-исследовательский институтAgrophysical Research InstituteАгрофизический научно-исследовательский институтAl al-Bayt UniversityАль-Байт Университет09082021101020211932092171905202109082021Copyright ©; 2021,Copyright ©; 2021, ООО "Эко-Вектор"2021ООО "Эко-Вектор"https://journals.eco-vector.com/ecolgenet/article/view/70759

Surface of oil-contaminated soil from Industrial Estate of Al-Mafraq city, Jordan, was investigated for the presence of aerobic oil-degrading moderately thermophilic bacteria. A pure culture of spore – forming aerobic chemoorganogeterotrophic rod shaped bacterial isolate, designated as strain j3n, was obtained. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain j3n is closely related to gram-positive bacteria of kaustophilus – thermoleovorans cluster of Geobacillus genus. Strain j3n grew aerobically with oil, hexadecane, benzoate and acetate. Growth data indicated that utilization of hexadecane but not of oil and benzoate might be under catabolite repression control. Possibility of a regulation of alkane degradation by acetate in aerobic thermophilic gram-positive bacteria of Geobacillus spp. was shown for the first time.

Поверхность нефтезагрязненной почвы промышленной зоны г. Аль-Мафрак (Иордания) была исследована на предмет выявления присутствия аэробных нефтеокисляющих умеренно термофильных бактерий. Чистая культура спорообразующих аэробных хемоорганогетеротрофных бактерий, штамм j3n, была выделена из одной пробы. Филогенетический анализ последовательности нуклеотидов гена 16S рРНК показал, что штамм j3n принадлежит к грамположительным бактериям группы kaustophilus – thermoleovorans рода Geobacillus. Особенности роста штамма в средах с разными субстратами показали, что использование гексадекана, но не нефти и бензоата, в присутствии ацетата могло контролироваться механизмом катаболитной репрессии. Возможность регулирования разложения алканов ацетатом с участием этого механизма аэробными термофильными грамположительными бактериями Geobacillus spp. показана в первый раз.

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