Vol 13, No 1 (2015)

Pichia pastoris yeasts are widely used as a production platform for heterologous proteins. Wide biotechnological use of these yeasts is determined by simplicity of cultivation, cheap media and ability to provide posttranslational modifications. Basic approaches for enhancement of the recombinant protein outcome constitue increasing number of gene copies, which encode target protein, as well as co-expression of supporting factors for protein folding, processing and secretion. Development of relevant plasmids and auxotrophic strains is essential for solving these tasks. In this study, we report plasmids and strains collection, whichwill allow to conduct integration of multiple foreign genes in P. pastoris genome

Respiratory diseases are among the leading causes of infant morbidity. Disturbances of functioning of the immune system play an important role in their development. Interleukin-10 (IL-10) is a key regulator of the immune response. Mononucleotide substitutions at positions (-1082), (-819) and (-592) of IL-10 gene results in low level of the protein production. Our purpose was to study the associations between polymorphic markers of IL-10 gene and chronic respiratory diseases in children living under conditions of anthropogenic pressure. 189 adolescents living in a city with high levels of air pollution and 82 from a city with a moderate level of contamination were examined. Children with chronic upper airway pathology in remission were identified. Blood samples from all children were tested for allelic variants -1082G / A, -592C / A, -819C / T of IL-10 gene in. Analysis of associations between polymorphic variants and the presence of chronic respiratory diseases was conducted. The -592C allele of IL-10 gene was less common among children with chronic diseases of the respiratory tract living in conditions of moderate air pollution than in the healthy comparison group. Similar association has not been established in thr group of children living in conditions of high air pollution. Thus, the C allele of the polymorphic -592C/A locus marks resistance to the development of a chronic disease of the upper respiratory tract in children living in conditions of moderate air pollution, while in conditions of high level of pollution contribution of genetic factors in its development is leveled.