Interleukins 4 and 6 as factors of modulation of subpopulation composition of blood monocytes in patients with ischemic cardiomyopathy

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Abstract


Aim. To evaluate the ratio of the fractions of classical, intermediate, non-classical and transitional monocytes in correlation with the concentration of interleukins 4 and 6 in the blood of patients with ischemic cardiomyopathy.

Methods. 18 patients with ischemic cardiomyopathy (17 men and 1 woman) aged 47-66 years with circulatory insufficiency of functional class II-III according to the classification of heart failure of the New York Heart Association, were examined. The control group included 14 healthy donors matched by gender and age to patients with ischemic cardiomyopathy without any diseases of cardiovascular system and other systems in an exacerbation stage. In blood of the patients with ischemic cardiomyopathy, the relative content of classical (CD14++CD16-), intermediate (CD14++CD16+), non-classical (CD14+CD16+) and transitional (CD14+CD16-) monocytes was assessed by flow cytometry and the concentration of interleukins 4 and 6 by enzyme-linked immunosorbent assay (ELISA).

Results. It was shown that the number of non-classical monocytes in the blood of patients with ischemic cardiomyopathy was 2 times lower than normal (5.05 % [4.08; 6.58] and 10.07 % [9.34; 13.84], respectively, p < 0.01), as well as the concentration of interleukin-4 (0.02 pg/ml [0; 0.04] and 0.15 pg/ml [0.05; 0.65], respectively, p < 0.05). The number of classical monocytes in the blood of patients had a tendency to decrease, and the proportion of intermediate monocytes and the concentration of interleukin-6, on the contrary, were slightly higher than in healthy individuals, and were interdependent (r = 0.61; p < 0.05). The relative content of transitional monocytes in the blood was comparable with that of healthy donors.

Conclusions. The subpopulation composition of blood monocytes in patients with ischemic cardiomyopathy is characterized by a deficiency of the fraction of non-classical monocytes with protective properties against endothelium, and interleukin-4 in the blood with a certain increase in the content of interleukin-6 and the number of intermediate cells with ability to cooperate with T-lymphocytes, which predisposes to diffuse atheromatosis of small coronary arteries and diffuse hypoxic myocardial damage in ischemic cardiomyopathy.


D A Azarova

Siberian State Medical University

Author for correspondence.
Email: azarova_d_a@mail.ru
Tomsk, Russia

S P Chumakova

Siberian State Medical University

Email: azarova_d_a@mail.ru
Tomsk, Russia

O I Urazova

Siberian State Medical University

Email: azarova_d_a@mail.ru
Tomsk, Russia

M V Vins

Siberian State Medical University

Email: azarova_d_a@mail.ru
Tomsk, Russia

V M Shipulin

Tomsk National Research Medical Center of RAS

Email: azarova_d_a@mail.ru
Tomsk, Russia

A S Pryakhin

Tomsk National Research Medical Center of RAS

Email: azarova_d_a@mail.ru
Tomsk, Russia

V V Novitskiy

Siberian State Medical University

Email: azarova_d_a@mail.ru
Tomsk, Russia

  • Richardson P., McKenna W., Bristow M., et al. Report of the 1995 World Health Organization / ­International Society and Federation of Cardiology Task Force on the Definitionand Classification of cardiomyopathies. Circulation. 1996; 93 (5): 841–842. doi: 10.1161/01.CIR.93.5.841.
  • Osovskaya N.Y. Ischemic cardiomyopathy: terminology, epidemiology, pathophysio­logy, diagnosis, therapeutic approach. Novosti meditsiny i farmacii. 2011; 359: 16–19. (In Russ.)
  • Bartekova M., Radosinska J., Jelemensky M., Dhalla N.S. Role of cytokines and inflammation in heart function during health and disease. Heart Fail. Rev. 2018; 23 (5): 733–758. doi: 10.1007/s10741-018-9716-x.
  • Lezhnjov A.A., Shipulin V.M., Krivoshhekov E.V. Postoperative left ventricular remodeling. Clinical and morphological aspects. LAP Lambert Academic Publishing. 2012; 152 р. (In Russ.)
  • Guddeti R.R., Matsuo Y., Matsuzawa Y., Kushwaha S.S., Lerman A. Ischemic cardiomyopathy is associated with coronary plaque progression and higher event rate in patients after cardiac transplantation. J. Am. Heart. Assoc. 2014; 3 (4): 10–91. doi: 10.1161/JAHA.114.001091.
  • Kovalenko V.N., Gavrish A.S. Ischemic cardiomyopathy: neurohumoral and immuno-inflammatory aspects of pato- and ­morphogenesis. Ukrainskii kardiologicheskii zhurnal. 2013; 4: ­14–20. (In Russ.)
  • Ziegler-Heitbrock L. The CD14+CD16+ blood monocytes: their role infection and inflammation. J. Leukoc. Biol. 2007; 3: 584–592. doi: 10.1189/jlb.0806510.
  • Ziegler-Heitbrock L., Hofer T.P.J. Toward a refined definition of monocyte subsets. Front. Immunol. 2013; 4: 23. doi: 10.3389/fimmu.2013.00023.
  • Matveeva V.G., Golovkin A.S., Kudryavtsev I.V., Grigoriev E.V., Chernova M.N. Dynamics of CD14+CD16+ monocytes subpopulations in complication-free systemic inflammatory response following coronary artery bypass graft surgery. Meditsinskaya immunologia. 2012; 14 (4–5): 391–398. (In Russ.)
  • Kazakov V.A., Shipulin V.M., Lezhnev A.A., Kozlov B.N., Krivoshhekov E.V., Suhodolo I.V., Gutor S.S. The clinic and morphological parallels postoperative remodeling of the left ventricle. Patologiya krovoobrasheniya i ­kardiohirurgia. 2009; 4: 23–27. (In Russ.)
  • Kazakov V.A., Suhodolo V.I., Shipulin V.M. Ultrastructural aspects of postinfarction remodeling of the left ventricle. Sibirskii meditsinskiy ­zhurnal. 2009; 4: 6–11. (In Russ.)
  • Italiani P., Boraschi D. From Monocytes to M1/M2 Macrophages: Phenotypical vs. Functional Differentiation. Front. Immunol. 2014; 5: 514. DOI: 10.3389/
  • fimmu.2014.00514.
  • Dutta P., Nahrendorf M. Monocytes in ­myocardial infarction. Arterioscler., Thromb., Vasc. Biol. 2015; 35: 1066–1070. doi: 10.1161/ATVBAHA.114.304652.
  • Barisionea C., Garibaldia S., Ghigliottia G., Fabbia P., Altieria P., Casalea M., Spallarossaa P., Berteroa G., Balbia M., Corsigliab L., Brunellia C. CD14CD16 monocyte subset levels in heart failure patients. Disease Marker. 2010; 28 (2): 115–124. doi: 10.3233/DMA-2010-0691.
  • Lilly L.S. Pathophysiology of the cardiovascular system. Ed. by L.S. Lilly; translated from English. M.: Binom. Knowledge laboratory. 2016: 735 р. (In Russ.)
  • Fadini G.P., Simoni F., Cappellari R., Previato L., Avogaro A. Pro-inflammatory monocyte-macrophage polarization imbalance in human hypercholesterolemia and atherosclerosis. Atherosclerosis. 2014; 237 (2): 805–808. doi: 10.1016/j.atherosclerosis.2014.10.106.
  • Jaipersad A.S., Shantsila A., Lip G.Y.H., Shantsila E. Expression of monocyte subsets and ­angiogenic markers in relation to carotid plaque neovascularization in patients with pre-existing coronary artery disease and carotid stenosis. Ann. Med. 2014; 46 (7): 530–538. doi: 10.3109/07853890.2014.931101.
  • Esper R.J. Hypertension arterial mechanics and ­other issues. Revista Argentina de Cardiologia. 2011; 79 (4): 13.

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© 2018 Azarova D.A., Chumakova S.P., Urazova O.I., Vins M.V., Shipulin V.M., Pryakhin A.S., Novitskiy V.V.

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