Assessment of the effect of exogenous fibrin monomer on post-traumatic bleeding in hypofibrinogenemia caused by administration of snake venom Agkistrodon rhodostoma
- Authors: Vdovin VM1,2, Momot AP1,3,2, Orekhov DA4, Shakhmatov II1,2, Lycheva NA2, Momot DA1
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Affiliations:
- Altai State Medical University
- State Research Institute of Physiology and Fundamental Medicine
- Altai Branch of FSBI, National Research Center for Hematology
- Altay Regional Dispensary of Cardiology
- Issue: Vol 101, No 5 (2020)
- Pages: 704-712
- Section: Experimental medicine
- Submitted: 18.06.2020
- Accepted: 15.09.2020
- Published: 27.10.2020
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/34776
- DOI: https://doi.org/10.17816/KMJ2020-704
- ID: 34776
Cite item
Abstract
Aim. To assess the effect of fibrin monomer on the rate of blood loss after controlled liver injury in hypofibrinogenemia induced by systemic administration of Malayan pit viper venom (Agkistrodon rhodostoma).
Methods. A placebo-controlled study of the hemostatic effect of fibrin monomer administered intravenously at 0.25 mg/kg, and coagulation parameters in the controlled liver injury with profound hypofibrinogenemia caused by administration of Malayan pit viper venom was conducted in 34 male Chinchilla rabbits. The distribution of the studied parameters was investigated by the Shapiro–Wilk test. Statistical differences between groups were tested by Student’s t-test, Mann–Whitney U test, or Wilcoxon test, as appropriate. Differences in mortality rate were examined using Fisher's exact test.
Results. A model of experimental toxogenic disseminated intravascular coagulation was reproduced, manifested by high mortality of animals (50.0%), severe blood loss (increased blood loss by 1.78 times), hemolysis, a decreased platelet count (by 19.6% of median) and platelet dysfunction, fibrinogen consumption (protein content less than 0.9 g/l), hypocoagulation as well as intensive D-dimer production (increased concentration by 25.0 times of median). A high level of the fibrin derivative demonstrated activation of fibrin formation and fibrinolysis in the bloodstream of the animals. Systemic prophylactic administration of exogenous fibrin monomer after receiving snake venom did not lead to a decrease in post-traumatic bleeding, whereas earlier, during reproduction of disseminated intravascular coagulation caused by streptokinase infusion, such a hemostatic effect of fibrin monomer was shown.
Conclusion. The absence of fibrin monomer effect (at a dose of 0.25 mg/kg) on the severity of blood loss in toxogenic disseminated intravascular coagulation may be associated with more profound disseminated intravascular coagulation and a sharp 25-fold increase in D-dimer levels that can act as a fibrin monomer polymerization inhibitor.
Full Text
About the authors
V M Vdovin
Altai State Medical University; State Research Institute of Physiology and Fundamental Medicine
Author for correspondence.
Email: erytrab@gmail.com
Russian Federation, Barnaul, Russia; Novosibirsk, Russia
A P Momot
Altai State Medical University; Altai Branch of FSBI, National Research Center for Hematology; State Research Institute of Physiology and Fundamental Medicine
Email: erytrab@gmail.com
Russian Federation, Barnaul, Russia; Barnaul, Russia; Novosibirsk, Russia
D A Orekhov
Altay Regional Dispensary of Cardiology
Email: erytrab@gmail.com
Russian Federation, Barnaul, Russia
I I Shakhmatov
Altai State Medical University; State Research Institute of Physiology and Fundamental Medicine
Email: erytrab@gmail.com
Russian Federation, Barnaul, Russia; Novosibirsk, Russia
N A Lycheva
State Research Institute of Physiology and Fundamental Medicine
Email: erytrab@gmail.com
Russian Federation, Novosibirsk, Russia
D A Momot
Altai State Medical University
Email: erytrab@gmail.com
Russian Federation, Barnaul, Russia
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