Effect of chronic stress on the relative level of dopamine receptor gene expression
- Authors: Valeeva E.V.1,2, Semina I.I.2, Galeeva A.G.1,3, Mukhametshina A.D.1, Mukhametshina R.D.1, Kravtsova O.A.1
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Affiliations:
- Kazan (Volga Region) Federal university
- Kazan state medical university
- Federal Center for toxicological, radiation, and biological safety — Federal Research Veterinary Institute
- Issue: Vol 103, No 3 (2022)
- Pages: 418-426
- Section: Experimental medicine
- Submitted: 02.08.2021
- Accepted: 04.05.2022
- Published: 09.06.2022
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/77258
- DOI: https://doi.org/10.17816/KMJ2022-418
- ID: 77258
Cite item
Abstract
Background. The regulation of the central dopaminergic system under the influence of chronic stress is disturbed, however, the dynamics of changes in the dopamine receptors expression in the periphery remains poorly understood.
Aim. Evaluation of the different models of chronic stress influence on changes in the relative level of dopamine receptor gene expression in peripheral blood cells of rats during immobilization and intense physical activity.
Material and methods. For 270 days on 88 Wistar rats, the study on the effect of different models of chronic stress on the change in the relative level of Drd1–5 genes expression was performed in four groups: the first control group; the second group was subjected to intensive physical activity in the “Forced swimming with a load” test (7-minute swimming with a load of 8% of body weight 2 times a week); the third group experienced daily 90-minute immobilization for 14 days; the fourth group had combined exposure of physical activity and immobilization. The relative level of dopamine receptor gene expression was determined by real-time polymerase chain reaction after 90, 180, and 270 days of the experiment in peripheral blood cells of the tail vein. The calculation of the relative level of gene expression was carried out based on the Livak method (2–ΔΔCt); the assessment of the difference significance — using a two-sample t-test for independent samples.
Results. The analysis of the relative level of genes encoding D1-type dopamine receptors expression showed that a decrease in the Drd1 gene expression level after 90 days of the experiment was detected only in male rats from immobilization stress and control groups [RQ 0.35 (p=0.003) and 0.21 (p=0.002), respectively], while in males from other groups and females, the activity of this gene did not change significantly throughout the course of the experiment. The relative expression level of Drd5 gene changed only in female rats. In females subjected to intense physical activity, the level of this gene expression increased almost 4 times (RQ 3.82, p=0.005) 90 days after the start of the experiment, and in females of the control group, the transcriptional activity of the gene decreased 4 times after 180 days of the experiment (RQ 0.25, p=0.015). When assessing changes in the activity of genes encoding D2-type receptors for the Drd3 and Drd4 genes, a significant increase in the relative expression level was revealed in all experimental groups, both in males and females, on the 180th day of exposure to stress factors. At the same time, activation of both genes was occurred after 90 days in the control group only in females and persisted up to another 90 days, after which it returned to the initial level. Expression of the Drd2 gene wasn't detected in rat blood cells.
Conclusion. The relative level of expression of D1- and D2-like receptor genes in rat peripheral blood cells depends on the type of chronic stress and has pronounced sexual dimorphism.
Full Text
About the authors
Elena V. Valeeva
Kazan (Volga Region) Federal university; Kazan state medical university
Author for correspondence.
Email: vevaleeva@ya.ru
ORCID iD: 0000-0001-7080-3878
SPIN-code: 4670-8980
Scopus Author ID: 57195580617
ResearcherId: W-8036-2019
Junior Researcher, Central research laboratory, Kazan State Medical University; Assistant, Depart. of Biochemistry, Biotechnology and Pharmacology
Russian Federation, Kazan, Russia; Kazan, RussiaIrina I. Semina
Kazan state medical university
Email: seminai@mail.ru
ORCID iD: 0000-0003-3515-0845
Prof., Depart. of Pharmacology, Head, Central research laboratory
Russian Federation, Kazan, RussiaAntonina G. Galeeva
Kazan (Volga Region) Federal university; Federal Center for toxicological, radiation, and biological safety — Federal Research Veterinary Institute
Email: antonina-95@yandex.ru
ORCID iD: 0000-0003-2650-6459
Cand. Sci. (Vet.), Junior Researcher, Open Lab “Markers of Pathogenesis”; Junior Researcher
Russian Federation, Kazan, Russia; Kazan, RussiaAlbina D. Mukhametshina
Kazan (Volga Region) Federal university
Email: albinam1709@gmail.com
ORCID iD: 0000-0002-5296-1861
Bachelor, Depart. of Biochemistry, Biotechnology and Pharmacology
Russian Federation, Kazan, RussiaRegina D. Mukhametshina
Kazan (Volga Region) Federal university
Email: 1709mrd@gmail.com
ORCID iD: 0000-0002-5797-993X
Bachelor, Depart. of Biochemistry, Biotechnology and Pharmacology
Russian Federation, Kazan, RussiaOlga A. Kravtsova
Kazan (Volga Region) Federal university
Email: okravz@yandex.ru
ORCID iD: 0000-0002-4227-008X
Cand. Sci. (Biol.), Assoc. Prof., Depart. of Biochemistry, Biotechnology and Pharmacology
Russian Federation, Kazan, RussiaReferences
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