I.P. Pavlov Russian Medical Biological HeraldI.P. Pavlov Russian Medical Biological Herald0204-34752500-2546Eco-Vector485010.17816/PAVLOVJ2014242-48Research ArticleCHANGES IN BALANCE OF T-HELPER SUBSETS IN THE GALT OF RATS UNDER CHRONIC ZOOSOCIAL STRESS AND MODULATION OF THE INTESTINAL MICROFLORA15062014222424828102016Copyright © 2014, Topol I.A., Kamyshny A.M.2014It was investigated the influence of chronic zoosocial stress (ZSS) and modulation of the intestinal microflora composition on the ratio of T-helper subsets in GALT. It has been established that ZSS development was accompanied by an imbalance Tbet+/Gata3+- and Foxp3 +/Roryt +-cells, indicating the dominance of Th1- and Th17-differentiation and increasing levels of pro-inflammatory signaling in the gut. It was shown that the introduction of kanamycin to the stressed rats leads to the dominance of Th1- and Treg subsets, but modulation of the intestinal microflora lactobacterine reduces the ratio of Tbet +/Gata3 + - lymphocytes in the own lamina of mucous membrane of the fibres, increases in subepithelial zone and increases unidirectionally the ratio of Treg/Th17.TregstressGALTT-helper cellsTregстрессКАЛТT-хелперы[Avgustinovich D. Gender-related characteristics of responding to prolonged psychoemotional stress in mice / D. Avgustinovich, I. Kovalenko // Neurosc. Behav. Physiol. - 2009. - Vol. 40, № 3. -Р. 858-867.][Caton A. Regulatory cells in health and disease / A. Caton, K. Weissler // Immunol Rev. - 2014. - Vol. 259, №1. - P. 5-10.][Chronic psychosocial stress promotes systemic immune activation and the development of inflammatory Th17 cell responses / D. Schmidt [et al.] // Brain Behav Immun. - 2010. - Vol. 24, №7. -P. 1097-1104.][Cryan J. Mind-altering microorganisms: the impact of the gut microbiota on brain and behavior / J. Cryan, T. Dinan // NatureReviews Neuroscience. - 2012. -Vol. 9. - P. 1-12.][Evaluation of an improved automated analysis of freezing behavior in rats and its use in trace fear conditioning / A.R. Marchand [et al.] // J. Neurosci. Methods. - 2003. - Vol. 126. -Р. 145-153.][Host interactions with Segmented Filamentous Bacteria: An unusual trade-off that drives the post-natal maturation of the gut immune system / P. Schnupf [et al.] // Semin. Immunol. - 2013. -Vol. 25. - P. 342-351.][Imbalance between Th17 and T-reg cells may play an important role in the development of chronic unpredictable mild stress-induced depression in mice / M. Hong [et al.] // Neuroimmunomodulation. - 2013. - Vol. 20, №1. - P. 39-50.][Kaluyev A.V. Stress and grooming / A.V. Kaluyev. - M.: Aviks, 2002. - 146 p.][Littman D. Th17 and regulatory T cells in mediating and restraining inflammation / D. Littman, A. Rudensky // Cell. - 2010. - Vol. 140. - Р. 845-858.][Mucosal immunosuppression and epithelial barrier defects are key events in murine psychosocial stress-induced colitis / S. Reber [et al.] // Brain Behav. Immun. - 2011. - Vol. 25. - Р. 1153-1161.][Probioticsincrease T regulatory cells and reduceseverity of experimental colitis in mice / H. Zhao [et al.] // World J Gastroenterol. - 2013. - Vol. 19, № 5. -P. 742-749.][Psychosocial stress and inflammation in cancer / N. Powell [et al.] // Brain, Behavior and Immunity. - 2013. - Vol. 30. -Р. 41-47.][T-bet and Gata3 in controlling type 1 and type 2 immunity mediated by innate lymphoid cells / T. Hoyler [et al.] // Curr Opin Immunol. - 2013. - Vol. 25, № 2. -Р. 139-147.][T-bet: a bridge between innate and adaptive immunity / V. Lazarevic [et al.] // Nat Rev Immunol. - 2013. - Vol. 11. -Р. 777-789.][Well-controlled pro-inflammatory cytokine responses of Peyer’s patch cells to probiotic Lactobacillus casei / Y. Chiba [et al.] // Immunology. - 2010. - Vol. 130, №3. - P. 352-362.]