The effect of proinflammatory cytokine inhibtors on the course of aseptic necrosis of the femoral head in a model experiment
- Authors: Shabaldin N.1, Shabaldin A.V.1, Bogdanov L.A.2, Igisheva L.N.1,2, Zinchuk S.F.1, Chervov A.E.3
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Affiliations:
- Kemerovo State Medical University
- Institute for Complex Issues of Cardiovascular Diseases
- Kuzbass Clinical Pathology Bureau
- Issue: Vol 10, No 2 (2022)
- Pages: 171-182
- Section: Experimental and theoretical research
- Submitted: 26.01.2022
- Accepted: 14.04.2022
- Published: 30.06.2022
- URL: https://journals.eco-vector.com/turner/article/view/98467
- DOI: https://doi.org/10.17816/PTORS98467
- ID: 98467
Cite item
Abstract
BACKGROUND: One of the most relevant areas of study of Perthes’ disease is the search and scientific justification of new targets for the treatment of osteodestructive processes. At the same time, an important link in the regulation of bone homeostasis is the cytokine system that affects the processes of differentiation and activation of osteoclasts through the RANK-RANKL-OPG signaling pathway. Inhibition of the biological action of proinflammatory cytokines in the early stages of aseptic necrosis is a promising direction in the study of antiresorptive therapy.
АIM: To assess the effectiveness of monoclonal blockers of proinflammatory cytokines in the treatment of aseptic necrosis of the femoral head in a model experiment on Wistar rats.
MATERIALS AND METHODS: A model experiment was performed on small animals (24 Wistar rats). All animals underwent surgical induction of aseptic necrosis of the femoral head. The rats were divided into four groups: group 1 did not receive treatment, group 2 were administered a monoclonal IL-6 blocker, group 3 was administered a TNFα binding inhibitor, and group 4, a monoclonal IL-1β blocker. Moreover, histological, X-ray, biochemical, and immunological studies were equally performed.
RESULTS: We revealed the influence of monoclonal blockers of the biological action of proinflammatory cytokines on the processes of osteogenesis during the manifestation of aseptic necrosis of the femoral head. The most favorable outcomes of the course of aseptic necrosis of the femoral head in the form of greater preservation of the cartilaginous, bony structure were obtained in rats treated with a monoclonal blocker of the proinflammatory cytokine, IL-6.
CONCLUSIONS: Genetically engineered biological agents of receptor blockers of proinflammatory cytokines affect aseptic inflammation, especially during osteodestruction. Further studies of the properties of the IL-6 monoclonal blocker is a promising aspect in the study of its effect on bone metabolism disorders in osteolysis.
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About the authors
Nikita Shabaldin
Kemerovo State Medical University
Email: Shabaldin.nk@yandex.ru
ORCID iD: 0000-0001-8628-5649
SPIN-code: 6283-2581
Scopus Author ID: 57209570350
MD, PhD, Cand. Sci. (Med.)
Russian Federation, 22a Voroshilov str., Kemerovo, 650029Andrey V. Shabaldin
Kemerovo State Medical University
Email: weit2007@yandex.ru
ORCID iD: 0000-0002-8785-7896
SPIN-code: 5281-0065
MD, PhD, Dr. Sci. (Med.), Assistant Professor
Russian Federation, 22a Voroshilov str., Kemerovo, 650029Lev A. Bogdanov
Institute for Complex Issues of Cardiovascular Diseases
Email: bogdanovleone@gmail.com
ORCID iD: 0000-0003-4124-2316
SPIN-code: 9547-9196
ResearcherId: R-9744-2018
MD, Junior Research Associate
Russian Federation, 22a Voroshilov str., Kemerovo, 650029Liudmila N. Igisheva
Kemerovo State Medical University; Institute for Complex Issues of Cardiovascular Diseases
Email: igisheval@yandex.ru
ORCID iD: 0000-0002-7102-3571
SPIN-code: 3025-1607
MD, PhD, Dr. Sci. (Med.)
Russian Federation, 22a Voroshilov str., Kemerovo, 650029; KemerovoSergei F. Zinchuk
Kemerovo State Medical University
Email: zinchuk.sf@kemsma.ru
ORCID iD: 0000-0001-7774-4196
SPIN-code: 3199-8459
MD, PhD, Cand. Sci. (Med.), Assistant Professor
Russian Federation, 22a Voroshilov str., Kemerovo, 650029Aleksandr E. Chervov
Kuzbass Clinical Pathology Bureau
Author for correspondence.
Email: acheront999@gmail.com
ORCID iD: 0000-0001-6168-4483
MD, pathologist
Russian Federation, 22a Voroshilov str., Kemerovo, 650029References
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