The influence of phlomoides tuberosa extract on structure of spleen in immunosupressive mice

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Abstract


Background. The immune system is extremely sensitive to adverse external and internal factors. In this context, a correction of immunosuppressive states of immune organs by means of complex and safe means, such as the extraction of medicinal plants is required. In this regard, plants containing phenylpropanoids are of interest. It was shown that the extract from Phlomoides tuberosa L. Moench showed immunostimulatory activity due to the content phenylpropanoids.

Purpose. The present study was aimed at the estimation of the effect of P. tuberosa extract оn the spleen structure using experimental azathioprine immunosupression model.

Materials and methods. The experiments were carried out on CBA male mice, 20-22 g of weight. Immune deficiency was modeled by the intragastrical introduction of azathioprine in the dose 50 mg/kg once a day for 5 days. The experimantal group of animals received the tested remedy once a day intragastrically in dose 1 mg/kg for 14 days against the background of azathioprine immunosupression. The intact group received the purified water according to the analogous scheme. Data were obtained using histological, morphometric and statistical analysis.

Results. The results have shown that azathioprine caused disruptive changes in the structure of the lymphoid tissue of the spleen: area of white pulp and the thickness of the periarterial lymphoid sheath (PALS) were equally less – in 1.7 times, the diameters of the lymphoid nodules and germinative centers were lower, respectively in 2.6 and 1.7 times, compared with a negative control. The course administration of the test remedy to animals was significantly reduced suppressive effect of cytostatic azathioprine: area of white pulp and the thickness PALS spleens of mice treated with the extract were 1.5 and 1.4 times the diameters of lymphoid nodules and germinative centers was 2.1 times and 1.2 times more positive control.

Conclusion. Thus, the data obtained allow to conclude that the dry extract P. tuberosa has the marked immune modulating activity that substantiates the expediency of its further study in order to create new effective immune modulators.


Darima Z. Tsyrenova

Author for correspondence.
dtsyrenova@mail.ru
Institute of General and Experimental Biology SB RAS
Russian Federation, Ulan-Ude, Russia

post-graduate student, Laboratory of Experimental Pharmacology

Sergey M. Gulyaev

s-gulyaev@inbox.ru
Institute of General and Experimental Biology SB RAS
Russian Federation, Ulan-Ude, Russia

PhD, Senior staff scientist, Laboratory of Safety of Biologically Active Substances

Valentina B. Khobrakova

val0808@mail.ru
Institute of General and Experimental Biology SB RAS
Russian Federation, Ulan-Ude, Russia

Dr, Senior staff scientist, Laboratory of Experimental Pharmacology

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