Predator odor induces genome instability in the mouse bone marrow cells

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Abstract


Background. Long coevolution of prey and predator species of mammals creates specific mechanisms of their interaction, e. g. prey’s innate behavior aversive to the predator odor. However, little is known about genetic responses in the prey organism. We assessed genome instability of the bone marrow cells in mice affected by the cat’s odor influence, and proposed pathway of such action.

Materials and methods. CBA mouse males were exposed to volatiles from adult cat urine for 2 or 24 hours. To estimate the genetic effect, ana-telophase method of chromosome aberration analysis and comet assay were used. The level of corticosterone was also measured after the exposure for 30 or 60 minutes.

Results. The exposure to cat’s urine volatiles for 2 hours induced damage of DNA in bone marrow cells of the mouse males as was shown by the DNA comet analysis. The exposure for 24 hours elevated the frequency of chromosome aberrations in mitotically dividing cells at ana-telophase stage. No significant changes were found in the level of corticosterone in the peripheral blood.

Conclusion. We have shown that volatile chemosignals from predator’s urine induce genomic instability in bone marrow cells of a prey. The hormonal pathway of such influence is still unknown. Intraorganismic paths leading to genome damage are discussed as well as far consequences of discovered effects.


Timofey S Glinin

Author for correspondence.
t.glinin@gmail.com
St Petersburg State University
Russian Federation, Saint Petersburg, Russia

PhD student. Department of Genetics and Biotechnology

Polina A Starshova

pstrsh@gmail.com
St Petersburg State University
Russian Federation, Saint Petersburg, Russia

student. Department of Genetics and Biotechnology

Victoria A Shubina

vicktory.shubina@gmail.com
St Petersburg State University
Russian Federation, Saint Petersburg, Russia

student. Department of Genetics and Biotechnology

Margarita V Anisimova

anisimova@bionet.nsc.ru
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Russian Federation, Novosibirsk, Russia

PhD student. Department of Genetic Resources of Experimental Animals

Anton A Bondarenko

emptybox1267@gmail.com
St Petersburg State University
Russian Federation, Saint Petersburg, Russia

student. Department of Genetics and Biotechnology

Mikhail P Moshkin

mmp@bionet.nsc.ru
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Russian Federation, Novosibirsk, Russia

Dr. of Sci. Department of Genetic Resources of Experimental Animals

Eugene V Daev

mouse_gene@mail.ru
St Petersburg State University
Russian Federation, Saint Petersburg, Russia

Professor, Department of Genetics and Biotechnology

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Supplementary files

Supplementary Files Action
1. Fig. 1. (A) The total frequency of damaged cells in bone marrow of mouse CBA males with percentage of DNA in the comet tail >5%. (B) The frequency of “slightly damaged” cells with percentage of DNA in the comet tail 5-20%. (C) The frequency of “highly damaged” cells with percentage of DNA in the comet tail >20%. Variants of treatment: CU2 and CU24 - olfactory exposure with cat urine for 2 and 24 hours; C - control olfactory exposure with water; AA – acrylamide injection. The median, first and third quartiles, the minimum and the maximum values are shown (comparisons by Mann–Whitney test). View (18KB) Indexing metadata
2. Fig. 2. Total chromosome aberration frequency (Mean ± 95%SD) in dividing bone marrow cells of mouse CBA males after 24 hour treatment by volatile cat urine chemosignals. C – control treatment with water, CU24 - 24 hour exposure with cat urine volatile chemosignals, N – number of animals in group (comparisons by t-test). View (7KB) Indexing metadata
3. Fig. 3. The level of whole plasma corticosterone in mouse CBA males after treatment by volatile compounds of cat urine. C- control; CU30 and CU60 – 30 and 60 minutes exposure with cat urine volatile chemosignals respectively. The median, first and third quartiles, the minimum and the maximum values are shown (Mann–Whitney test , p > 0,1). View (17KB) Indexing metadata

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Copyright (c) 2017 Glinin T.S., Starshova P.A., Shubina V.A., Anisimova M.V., Bondarenko A.A., Moshkin M.P., Daev E.V.

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