Effect of crosslinking with riboflavin and ultraviolet a (UVA) on the scleral tissue structure

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Abstract


Purpose. To evaluate the effect of the scleral crosslinking with riboflavin and ultraviolet A (UVA) on the scleral tissue structure in vitro experiments.

Material and methods. The study was conducted on 7 porcine cadaver eyes. Two parallel scleral strips were excised from each eyeball, one of which was subjected to crosslinking procedure (instillation of 0.1% aqueous solution of riboflavin mononucleotide for 20 minutes, ultraviolet irradiation of 3 mW /cm2 for 30 minutes), second was used as control. Scleral structure was evaluated by light (Van Gieson’s stain) and electron microscopy. Morphometric analysis of the microphotographs was performed using special software.

Results. As a result of crosslinking, the average packing density of collagen fibers increased by 8.2%, the area of the intermediate space decreased by 5.2%, the average diameter of collagen fibrils increased by 12%. There were no pathological changes in scleral structures.

Conclusion. Obtained results confirm the efficacy of scleral crosslinking with riboflavin/UVA in the formation of additional cross links, and the procedure safety for the scleral tissue.


Mukharram M Bikbov

Author for correspondence.
eye@anrb.ru
Ufa Eye Research Institute
Russian Federation, Ufa, Russia

DMedSc, professor, director

Valentina K Surkova

ufaeyenauka@mail.ru
Ufa Eye Research Institute
Russian Federation, Ufa, Russia

DMedSc, professor, leading scientific researcher of the Department of the cornea and lens surgery

Emin L Usubov

emines.us@inbox.ru
Ufa Eye Research Institute
Russian Federation, Ufa, Russia

PhD, leading scientific researcher of the Department of the cornea and lens surgery

Nikolaj A Nikitin

nic@ufanet.ru
Ufa Eye Research Institute
Russian Federation, Ufa, Russia

PhD, leading scientific researcher of the Department of the cornea and lens surgery

Mikhail Nikolaevich Astrelin

astrelin87@yandex.ru
Ufa Eye Research Institute
Russian Federation, Ufa, Russia

scientific researcher of the Department of the cornea and lens surgery

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