Comparative analysis of the nitrogen monoxide content and optical-morphometric characteristics of erythrocyte-containing blood components during storage

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Flow cytometry was used to analyze the content of intracellular nitrogen monoxide in erythrocyte-containing blood components during blood storage with hemopreservative for 4 weeks. It was found that storing of blood is accompanied by increased levels of intracellular nitrogen monoxide in erythrocytes and thus by a change in optical and morphometric parameters of red blood cells. In the early stages of storage, erythrocytes were represented by discocytes, and during long storage erythrocytes became more spherical (spherocytes) as the percentage of microcytes that accumulated nitrogen monoxide increased and their intracellular hemoglobin levels were decreased.

作者简介

N. Akulich

National Antidoping Laboratory

Email: akulichn@gmail.com
Minsk Region, Belarus

V. Zinchuk

Grodno State Medical University

Grodno, Belarus

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