Biological Microarray for Detection of Somatic Mutations in the Genes of Isocitrate Dehydrogenase 1 and 2

V. O. Varachev; Варачев В. О.; D. A. Guskov; Гуськов Д. А.; A. P. Shekhtman; Шехтман А. П.; D. V. Rogozhin; Рогожин Д. В.; S. A. Polyakov; Поляков С. А.; A. V. Chudinov; Чудинов А. В.; A. S. Zasedatelev; Заседателев А. С.; T. V. Nasedkina; Наседкина Т. В.

doi:10.31857/S0132342323050093

Biological Microarray for Detection of Somatic Mutations in the Genes of Isocitrate Dehydrogenase 1 and 2

Authors: Varachev V.O.¹, Guskov D.A.¹, Shekhtman A.P.², Rogozhin D.V.², Polyakov S.A.¹, Chudinov A.V.¹, Zasedatelev A.S.¹, Nasedkina T.V.¹
Affiliations:
1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
2. Russian Children’s Clinical Hospital
Issue: Vol 49, No 5 (2023)
Pages: 537-542
Section: ПИСЬМА РЕДАКТОРУ
URL: https://journals.eco-vector.com/0132-3423/article/view/670571
DOI: https://doi.org/10.31857/S0132342323050093
EDN: https://elibrary.ru/ABXFZT
ID: 670571

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Abstract

Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) genes are important diagnostic and prognostic markers in a number of tumors (chondroid tumors, brain tumors, leukemia). To determine mutations in the IDH1/2 genes the method of allele-specific hybridization on a biological microarray was developed, which makes it possible to determine the presence of a mutation in a tumor sample (chondroid tumors and glioblastoma) and the variant of nucleotide substitution. When comparing IDH1/2 mutation detection methods using a biological microarray and DNA melting analysis with a TaqMan probe, full coincidence of results was obtained, with the biochip method making it possible to determine the type of nucleotide substitution without an additional sequencing step.

Keywords

isocitrate dehydrogenase 1 and 2, mutations, glioblastoma, chondroid tumors, diagnosis, biological microarray

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