Characteristics of the pro-regenerative effect of a bioplastic matrix based on a stabilized extracellular matrix: an experimental study

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Objective. To evaluate the regenerative potential of a bioplastic material (BPM) based on a soluble form of a stabilized extracellular matrix.

Materials and methods. Using light and fluorescence microscopy, we assessed the morphometric characteristics of cells, using flow cytometry and commercial kits, we assessed the expression of cell differentiation markers (CD16, CD14). Modeling of severe thermal burns was carried out on laboratory rats, in accordance with ethical principles (principles of "3R": replacement, reduction, refinement) under general anesthesia to reduce stress and pain.

Results. BPM is a porous film, the sorption capacity of BPM is 3.5 mg/mg. It was found that when co-incubated with human fibroblasts, BPM does not have a cytotoxic effect. It was revealed that the cultivation of BPM by human peripheral blood monocytes (PBMCh) prevents spontaneous activation of PBMCh into a proinflammatory phenotype. In vivo, it was shown that BPM promotes skin restoration in experimental rats after thermal injury.

Conclusion. Wound dressing based on a soluble form of stabilized extracellular matrix inhibits spontaneous activation of monocytes and their differentiation into a proinflammatory phenotype, stimulates the regeneration of tissues damaged by a thermal burn. At the same time, the effectiveness of the biomaterial exceeds therapy using anti-inflammatory ointment.

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作者简介

P. Markov

National Medical Research Center for Rehabilitation and Balneology

编辑信件的主要联系方式.
Email: p.a.markov@mail.ru
ORCID iD: 0000-0002-4803-4803

Candidate of Biological Sciences

俄罗斯联邦, Moscow

P. Eremin

National Medical Research Center for Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0001-8832-8470
俄罗斯联邦, Moscow

E. Rozhkova

National Medical Research Center for Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0002-2440-9244

Biol. D.

俄罗斯联邦, Moscow

I. Gilmutdinova

National Medical Research Center for Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0001-6743-2615

Candidate of Medical Sciences

俄罗斯联邦, Moscow

L. Marchenkova

National Medical Research Center for Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0003-1886-124X

MD

俄罗斯联邦, Moscow

参考

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2. Fig. 1. Mass of bioactive bioplastic material during incubation in Hartmann's solution (M ± SD; n = 7)

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3. Fig. 2. Protein extraction during incubation of bioactive bioplastic material in Hartmann's solution (М ± SD; n = 7)

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4. Fig. 3. Effect of bioplastic material on the number of living, apoptotic and dead cells after 48 hours of co-incubation (n = 7)

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5. Fig. 4. Expression of CD14 and CD16 receptors on PBMCh after 48 hours of incubation with bioplastic material (n = 5)

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6. Fig. 5. The area of damage to the skin of rats after induced thermal burn; Me (Q1; Q3) (n = 10)

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