Characteristics of the pro-regenerative effect of a bioplastic matrix based on a stabilized extracellular matrix: an experimental study

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Abstract

Objective. To evaluate the regenerative potential of a bioplastic material (BPM) based on a soluble form of a stabilized extracellular matrix.

Materials and methods. Using light and fluorescence microscopy, we assessed the morphometric characteristics of cells, using flow cytometry and commercial kits, we assessed the expression of cell differentiation markers (CD16, CD14). Modeling of severe thermal burns was carried out on laboratory rats, in accordance with ethical principles (principles of "3R": replacement, reduction, refinement) under general anesthesia to reduce stress and pain.

Results. BPM is a porous film, the sorption capacity of BPM is 3.5 mg/mg. It was found that when co-incubated with human fibroblasts, BPM does not have a cytotoxic effect. It was revealed that the cultivation of BPM by human peripheral blood monocytes (PBMCh) prevents spontaneous activation of PBMCh into a proinflammatory phenotype. In vivo, it was shown that BPM promotes skin restoration in experimental rats after thermal injury.

Conclusion. Wound dressing based on a soluble form of stabilized extracellular matrix inhibits spontaneous activation of monocytes and their differentiation into a proinflammatory phenotype, stimulates the regeneration of tissues damaged by a thermal burn. At the same time, the effectiveness of the biomaterial exceeds therapy using anti-inflammatory ointment.

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About the authors

P. A. Markov

National Medical Research Center for Rehabilitation and Balneology

Author for correspondence.
Email: p.a.markov@mail.ru
ORCID iD: 0000-0002-4803-4803

Candidate of Biological Sciences

Russian Federation, Moscow

P. S. Eremin

National Medical Research Center for Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0001-8832-8470
Russian Federation, Moscow

E. A. Rozhkova

National Medical Research Center for Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0002-2440-9244

Biol. D.

Russian Federation, Moscow

I. R. Gilmutdinova

National Medical Research Center for Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0001-6743-2615

Candidate of Medical Sciences

Russian Federation, Moscow

L. A. Marchenkova

National Medical Research Center for Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0003-1886-124X

MD

Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Mass of bioactive bioplastic material during incubation in Hartmann's solution (M ± SD; n = 7)

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3. Fig. 2. Protein extraction during incubation of bioactive bioplastic material in Hartmann's solution (М ± SD; n = 7)

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4. Fig. 3. Effect of bioplastic material on the number of living, apoptotic and dead cells after 48 hours of co-incubation (n = 7)

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5. Fig. 4. Expression of CD14 and CD16 receptors on PBMCh after 48 hours of incubation with bioplastic material (n = 5)

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6. Fig. 5. The area of damage to the skin of rats after induced thermal burn; Me (Q1; Q3) (n = 10)

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