Comparative analysis of quantitative polymerase chain reaction and culture methods for diagnosing urogenital infections and assessing vaginal microflora

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Modern diagnosis of infectious-inflammatory diseases and imbalances in the female urogenital tract microbiota requires selecting an optimal laboratory method. Based on a review of the literature, a comparative analysis of quantitative polymerase chain reaction and culture (microbiological) methods is conducted. The advantages and disadvantages of the methods are considered. Rational use of both methods, guided by their strengths and limitations, is key to effective clinical practice.

全文:

受限制的访问

作者简介

V. Kaptilnyy

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

编辑信件的主要联系方式.
Email: rtchilova@gmail.com
ORCID iD: 0000-0002-2656-132X
SPIN 代码: 4312-3455

Candidate of Medical Sciences, Professor

俄罗斯联邦

R. Chilova

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Email: rtchilova@gmail.com
ORCID iD: 0000-0001-6331-3109
SPIN 代码: 4137-4848

MD

俄罗斯联邦

D. Lystsev

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Email: rtchilova@gmail.com
ORCID iD: 0009-0006-3826-3174
俄罗斯联邦

M. Poznyak

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Email: rtchilova@gmail.com
ORCID iD: 0009-0006-8217-0784
俄罗斯联邦

参考

  1. Workowski K.A., Bachmann L.H., Chan P.A. et al. Sexually transmitted infections treatment guidelines, 2021. MMWR Recomm Rep. 2021; 70 (4): 1–187. doi: 10.15585/mmwr.rr7004a1
  2. Unemo M., Bradshaw C.S., Hocking J.S. et al. Sexually transmitted infections: challenges ahead. Lancet Infect Dis. 2017; 17 (8), e235-e279. doi: 10.1016/S1473-3099(17)30310-9
  3. Национальное руководство по акушерству. Под ред. Г.М. Савельевой, Г.Т. Сухих, В.Н. Серова, В.Е. Радзинского. 2-е изд., перераб. и доп. М.: ГЭОТАР-Медиа, 2022; 1080 с. [National Guidelines for Obstetrics. Ed. by G.M. Savelyeva, G.T. Sukhikh, V.N. Serov, V.E. Radzinsky. 2nd ed., revised and expanded. Moscow: GEOTAR-Media, 2022; 1080 pp. (in Russ.)].
  4. Национальное руководство по лабораторной диагностике. Под ред. В.В. Долгова, В.В. Меньшикова. М., 2013; 928 с. [National Guidelines for Laboratory Diagnostics. Edited by V.V. Dolgov, V.V. Menshikov. Moscow, 2013; 928 p. (in Russ.)].
  5. Chernesky M., Jang D., Luinstra K. et al. High analytical sensitivity and low rates of inhibition may contribute to detection of Chlamydia trachomatis in significantly more women by the APTIMA Combo 2 assay. J Clin Microbiol. 2006; 44 (2): 400–5. doi: 10.1128/JCM.44.2.400-405.2006
  6. Schachter J., Chernesky M.A., Willis D.E. et al. Vaginal swabs are the specimens of choice when screening for Chlamydia trachomatis and Neisseria gonorrhoeae: results from a multicenter evaluation of the APTIMA assays for both infections. Sex Transm Dis. 2005; 32 (12): 725–8. doi: 10.1097/01.olq.0000190092.59482.96
  7. Unemo M., Shafer W.M. Antimicrobial resistance in Neisseria gonorrhoeae in the 21st century: past, evolution, and future. Clin Microbiol Rev. 2014; 27 (3): 587–613. doi: 10.1128/CMR.00010-14
  8. Van Der Pol B., Ferrero D.V., Buck-Barrington L. et al. Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. J Clin Microbiol. 2001; 39 (3): 1008–16. doi: 10.1128/JCM.39.3.1008-1016.2001
  9. Schwebke J.R., Hobbs M.M., Taylor S.N. et al. Molecular testing for Trichomonas vaginalis in women: results from a prospective U.S. clinical trial. J Clin Microbiol. 2011; 49 (12): 4106–11. doi: 10.1128/JCM.01291-11
  10. Huppert J.S., Mortensen J.E., Reed J.L. et al. Rapid antigen testing compares favorably with transcription-mediated amplification assay for the detection of Trichomonas vaginalis in young women. Clin Infect Dis. 2007; 45 (2): 194–8. doi: 10.1086/518851
  11. Jensen J.S., Cusini M., Gomberg M. et al. 2016 European guideline on Mycoplasma genitalium infections. J Eur Acad Dermatol Venereol. 2016; 30 (10): 1650–6. doi: 10.1111/jdv.13849
  12. Lis R., Rowhani-Rahbar A., Manhart L.E. (2015). Mycoplasma genitalium infection and female reproductive tract disease: a meta-analysis. Clin Infect Dis. 2015; 61 (3): 418–26. doi: 10.1093/cid/civ312
  13. Shipitsyna E., Roos A., Datcu R. et al. (2013). Composition of the vaginal microbiota in women of reproductive age – sensitive and specific molecular diagnosis of bacterial vaginosis is possible? PLoS One. 2013; 8 (4): e60670. doi: 10.1371/journal.pone.0060670
  14. Fredricks D.N., Fiedler T.L., Marrazzo J.M. (2005). Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med. 2005; 353 (18): 1899–911. doi: 10.1056/NEJMoa043802
  15. Rumyantseva T.A., Bellen G., Romanuk T.N. et al. Utility of microscopic techniques and quantitative real-time polymerase chain reaction for the diagnosis of vaginal microflora alterations. J Low Genit Tract Dis. 2015; 19 (2): 124–8. doi: 10.1097/LGT.0000000000000060
  16. Menard J.P., Mazouni C., Salem-Cherif I. et al. (2010). High vaginal concentrations of Atopobium vaginae and Gardnerella vaginalis in women undergoing preterm labor. Obstet Gynecol. 2010; 115 (1): 134–40. doi: 10.1097/AOG.0b013e3181c391d7
  17. Mushi M.F., Bader O., Taverne-Ghadwal L. et al. Crucial role of fluconazole-diffusion tests for the identification of Candida glabrata in resource-limited settings. Journal of Fungi. 2010; 2 (4): 29.
  18. Ahmad A., Khan A.U. Prevalence of Candida species and potential risk factors for vulvovaginal candidiasis in Aligarh, India. Eur J Obstet Gynecol Reprod Biol. 2009; 144 (1): 68–71. doi: 10.1016/j.ejogrb.2008.12.020

补充文件

附件文件
动作
1. JATS XML

版权所有 © Russkiy Vrach Publishing House, 2025