Investigation of β-defensin-2 in breast milk in infant atopic dermatitis


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Purpose. To assess the link between p-defensin-2concentration in breastmilk with the severity of AD in its debut in infants and young children. Materlals and methods. 25 samples of breastmilk were analyzed. 2 groups were selected. The first group included 14 samples from mothers whose children got AD in the last 6 months: mild AD and moderate AD. Analysis of breastmilk samples by the enzyme-linked immunosorbent assay was conducted to find the p-defensin-2 concentration. ELISA Kit for p-Defensin-2 (DEFb2) was used. Results and dlscusslon. We have showed that in the breastmilk of healthy children mothers a background level of the p-defensin 2 is detected (209,6 pg/ml). In the case of mild AD the concentration of p-defensin 2 was lower (HBD-2 -166,3 pg/ml. In breastmilk of mothers whose children had moderate AD the level of p-defensin-2 concentration was significantly lower than in the first group (HBD-2 - 71,2 pg/ml). Thus we can make a conclusion that the decrease of the p-defensin-2 concentration in the breastmilk can be one of prognostic criteria of more severe AD in infants and young children.

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作者简介

Yu. Bunina (Boguslavskaya)

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Moscow

A. Kudryavtseva

I.M. Sechenov First Moscow State Medical University (Sechenov University); I.I. Mechnikov Research Institute for Vaccines and Sera

Email: kudassia@gmail.com
Professor, MD Moscow

E. Bystritskaya

I.I. Mechnikov Research Institute for Vaccines and Sera

Moscow

G. Usatova

I.I. Mechnikov Research Institute for Vaccines and Sera

Moscow

K. Levina

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Moscow

O. Svitich

I.M. Sechenov First Moscow State Medical University (Sechenov University); I.I. Mechnikov Research Institute for Vaccines and Sera

Moscow

参考

  1. Wollenberg A., Barbarot S., Bieber T. et al. Consensus based European guidelines for treatment of atopic eczema (atopic dermatitis) in adults and children: part I. J Eur Acad Dermatol Venereol. 2018; 32: 657-82. DOI: 10.1111/ jdv.14891
  2. Niyonsaba F., Nagaoka I., Ogawa H. et al. Multifunctional antimicrobial proteins and peptides: natural activators of immune systems. Curr Pharm Des. 2009; 15 (21): 2393-413. doi: 10.2174/138161209788682271
  3. Lee S.H., Jeong S.K., Ahn S.K. An update of the defensive barrier function of skin. Yonsei Med J. 2006; 47: 293-306. doi: 10.3349/ymj.2006.47.3.293
  4. Кудрявцева А.В., Катосова Л.К., Балаболкин И.П. и др. Роль золотистого стафилококка при атопическом дерматите у детей. Педиатрия. Журнал им. Г.Н. Сперанского. 2003; 6 (82): 32-6 [Kudryavtseva A.V., Katosova L.K., Balabolkin I.I. et al. Role of Staphylococcus aureus in pediatric atopic dermatitis. Pediatria. Journal named after G.N.Speransky. 2003; 82 (6): 32-6 (in Russ.)].
  5. Fleischer D.M., Spergel J.M., Assa A.H. et al. Primary prevention of allergic disease through nutritional interventions. J Allergy Clin Immunol Pract. 2008; 1: 29-36. doi: 10.1016/j.jaip.2012.09.003
  6. Gdalevich M., Mimouni D., David M. et al. Breast-feeding and the onset of atopic dermatitis in childhood: A systematic review and meta-analysis of prospective studies. J Am Acad Dermatol. 2001; 45: 520-7. doi: 10.1067/mjd.2001.114741
  7. Ito J., Fujiwara T. Breastfeeding and risk of atopic dermatitis up to the age 42 months: A birth cohort study in Japan. Ann Epidemiol. 2014; 24: 267-72. doi: 10.1016/j.annepidem.2013.11.007
  8. Munblit D., Treneva M., Peroni D.G. et al. Immune Components in Human Milk Are Associated with Early Infant Immunological Health Outcomes: A Prospective Three-Country Analysis. Nutrients. 2017; 9 (6): 532. doi: 10.3390/nu9060532
  9. Jakaitis B.M., Denning P.W. Human breast milk and the gastrointestinal innate immune system. Clin Perinatol. 2014; 41 (2): 423-35. DOI: 10.1016/j. clp.2014.02.011
  10. Hakansson A.P. Protective effects of human milk antimicrobial peptides against bacterial infection. J Pediatr (Rio J.). 2015; 91 (1): 4-5. https://doi. org/10.1016/j.jped.2014.10.001
  11. Metz-Boutigue M.-H., Shooshtarizadeh P., Prevost G. et al. Antimicrobial Peptides Present in Mammalian Skin and Gut are Multifunctional Defence Molecules. Curr Pharm Des. 2010; 16: 1024. https//doi.org/10.2174/138161210790963823,
  12. Tunzi C.R., Harper P.A., Bar-Oz B. et al. Beta-defensin expression in human mammary gland epithelia. Pediatr Res. 2000; 48: 30-5. doi: 10.1203/00006450200007000-00008
  13. Baricelli J., Rocafull M.A., Vazquez D. et al. p-Defensin-2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria. J Pediatr (Rio J.). 2015; 91 (1): 36-43. https://doi.org/10.1016/jjped.2014.05.006
  14. Jing Wu, Ruo-Lan Gong, Qing-Feng Hu et al. Immunoregulatory effect of human p-defensin 1 on neonatal cord blood monocyte-derived dendritic cells and T cells. Mol Immunol. 2019; 109: 99-107. https://doi.org/10.1016/j. molimm.2019.03.007.
  15. Savilahti E.M., Kukkonen A.K., Kuitunen M., et al. Soluble CD14, a- and p-defensins in breast milk: Association with the emergence of allergy in a high-risk population. Innate Immunity. 2015; 21 (3): 332-7. https://doi. org/10.1177/1753425914541560
  16. Rachid R., Chatila T.A. The role of the gut microbiota in food allergy. Curr Opin Pediatr. 2016; 6 (28): 748-75. doi: 10.1097/MOP.0000000000000427

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