Investigation of β-defensin-2 in breast milk in infant atopic dermatitis


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Abstract

Purpose. To assess the link between p-defensin-2concentration in breastmilk with the severity of AD in its debut in infants and young children. Materlals and methods. 25 samples of breastmilk were analyzed. 2 groups were selected. The first group included 14 samples from mothers whose children got AD in the last 6 months: mild AD and moderate AD. Analysis of breastmilk samples by the enzyme-linked immunosorbent assay was conducted to find the p-defensin-2 concentration. ELISA Kit for p-Defensin-2 (DEFb2) was used. Results and dlscusslon. We have showed that in the breastmilk of healthy children mothers a background level of the p-defensin 2 is detected (209,6 pg/ml). In the case of mild AD the concentration of p-defensin 2 was lower (HBD-2 -166,3 pg/ml. In breastmilk of mothers whose children had moderate AD the level of p-defensin-2 concentration was significantly lower than in the first group (HBD-2 - 71,2 pg/ml). Thus we can make a conclusion that the decrease of the p-defensin-2 concentration in the breastmilk can be one of prognostic criteria of more severe AD in infants and young children.

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About the authors

Yu. A Bunina (Boguslavskaya)

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Moscow

A. V Kudryavtseva

I.M. Sechenov First Moscow State Medical University (Sechenov University); I.I. Mechnikov Research Institute for Vaccines and Sera

Email: kudassia@gmail.com
Professor, MD Moscow

E. P Bystritskaya

I.I. Mechnikov Research Institute for Vaccines and Sera

Moscow

G. N Usatova

I.I. Mechnikov Research Institute for Vaccines and Sera

Moscow

K. Yu Levina

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Moscow

O. A Svitich

I.M. Sechenov First Moscow State Medical University (Sechenov University); I.I. Mechnikov Research Institute for Vaccines and Sera

Moscow

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