Biomimetic collagen-carragenan scaffold for non-drug stimulation of tissue regeneration

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Objective. To evaluate the mechanical strength, biodegradability and biocompatibility of composite hydro- and xerogels made from hydrolyzed type I collagen and kappa-carrageenan.

Materials and methods. Using the TA.XTplus texture analyzer, the strength of composite hydro- and xerogels, as well as the intensity of their biodegradation in acidic, neutral and alkaline environments, were estimated. Using light and fluorescence microscopy, the morphometric characteristics of human fibroblasts and their proliferative activity were estimated.

Results. It was found that carrageenan in the hydrogel increases the strength of the structure. Lyophilization of the G4C4-Hydro composite hydrogel allows obtaining a biomaterial with improved mechanical characteristics and increased resistance to destruction in acidic, neutral and alkaline salt solutions. It was found that the G4C4-Hydro and G4C4-Xero samples do not have an inhibitory effect on fibroblast proliferation. It was found that, unlike hydrogel, xerogels not only support fibroblast adhesion on their surface, but also provide favorable conditions that keep fibroblasts viable.

Conclusion. The composite xerogel G4C4-Xero containing hydrolyzed type I collagen and kappa-carrageenan can be used both as an extracellular scaffold for creating tissue-engineering structures and as an independent biomaterial for filling the volume of tissue lost as a result of surgery or injury.

全文:

受限制的访问

作者简介

P. Markov

National Medical Research Center of Rehabilitation and Balneology

编辑信件的主要联系方式.
Email: p.a.markov@mail.ru
ORCID iD: 0000-0002-4803-4803
SPIN 代码: 7493-5203

Candidate of Biological Sciences

俄罗斯联邦, Moscow

E. Rozhkova

National Medical Research Center of Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0002-2440-9244
SPIN 代码: 1578-6338

Biol. D.

俄罗斯联邦, Moscow

P. Eremin

National Medical Research Center of Rehabilitation and Balneology

Email: p.a.markov@mail.ru
ORCID iD: 0000-0001-8832-8470
SPIN 代码: 8597-6596
俄罗斯联邦, Moscow

参考

  1. Nasiri N., Haghdoost F., Habibi M. et al. Stem Cell Perspective for Regenerative Wound Healing: from Biology toward Future Clinical Directions: A Review. Cell Journal. 2025; 26 (10): 575–89. doi: 10.22074/cellj.2024.2034492.1613
  2. Pourjavadi A., Doroudian M., Ahadpour A. et al. Injectable chitosan/κ-carrageenan hydrogel designed with au nanoparticles: A conductive scaffold for tissue engineering demands. Int J Biol Macromol. 2019; 126: 310–7. doi: 10.1016/j.ijbiomac.2018.11.256
  3. Liu S., Yu J.-M., Gan Y.-C. et al. Biomimetic natural biomaterials for tissue engineering and regenerative medicine: new biosynthesis methods, recent advances, and emerging applications. Mil Med Res. 2023; 10 (1): 16. doi: 10.1186/s40779-023-00448-w.
  4. Amirrah I., Lokanathan Y., Zulkiflee I. et al. A comprehensive review on collagen type I development of biomaterials for tissue engineering: from biosynthesis to bioscaffold. Biomedicines. 2022; 10 (9): 2307. doi: 10.3390/biomedicines10092307
  5. Gu L., Shan T., Ma Y. et al. Novel Biomedical Applications of Crosslinked Collagen. Trends Biotechnol. 2019; 37 (5): 464–91. doi: 10.1016/j.tibtech.2018.10.007
  6. Farshidfar N., Iravani S., Varma R.S. Alginate-Based Biomaterials in Tissue Engineering and Regenerative Medicine. Marine Drugs. 2023; 21 (3): 189. doi: 10.3390/md21030189
  7. Klarak J., Brito A., Moreira L. et al. Using network analysis and large-language models to obtain a landscape of the literature on dressing materials for wound healing: The predominance of chitosan and other biomacromolecules: A review. Int J Biol Macromol. 2025; 26: 141565. doi: 10.1016/j.ijbiomac.2025.141565
  8. Pacheco-Quito E.M., Ruiz-Caro R., Veiga M.D. Carrageenan: Drug Delivery Systems and Other Biomedical Applications. Marine Drugs. 2020; 18 (11): 583. doi: 10.3390/md18110583
  9. Yegappan R., Selvaprithiviraj V., Amirthalingam S. et al. Carrageenan based hydrogels for drug delivery, tissue engineering and wound healing. Carbohydr Polym. 2018; 198: 385–400. doi: 10.1016/j.carbpol.2018.06.086
  10. Wolf M.T., Daly K.A., Brennan-Pierce E.P. et al. A hydrogel derived from decellularized dermal extracellular matrix. Biomaterials. 2012; 33 (29): 7028–38. doi: 10.1016/j.biomaterials.2012.06.051
  11. Drobnik J., Pietrucha K., Piera L. et al. Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture. Adv Clin Exp Med. 2017; 26 (2): 223–30. doi: 10.17219/acem/62835
  12. Jiang D., Rinkevich Y. Scars or Regeneration? Dermal Fibroblasts as Drivers of Diverse Skin Wound Responses. Int J Mol Sci. 2020; 21 (2): 617. doi: 10.3390/ijms21020617
  13. Katoh K. FAK-Dependent Cell Motility and Cell Elongation. Cells. 2020; 9 (1): 192. doi: 10.3390/cells9010192

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. Compressive strength of 4% gelatin hydrogel (G4), 4% carrageenan hydrogel (C4), composite hydrogel of 4% gelatin solution and 4% carrageenan solution (G4C4-Hydro) and composite xerogel of 4% gelatin solution and 4% carrageenan solution (G4C4-Xero) (the number of samples in each group is 5)

下载 (38KB)
索引源数据
3. Fig. 2. Strength of the composite hydrogel from 4% gelatin and 4% carrageenan solution (G4C4-Hydro) and the composite xerogel from 4% gelatin and 4% carrageenan solution (G4C4-Xero) after 24 hours of incubation in solution with acidic (pH = 6), neutral (pH = 7) and alkaline reactions (pH = 8) (the number of samples in each group is 5)

下载 (47KB)
索引源数据
4. Fig. 3. Human fibroblasts after 24 h of cultivation in a standard nutrient medium (a) and during co-cultivation with composite hydrogel G4C4-Hydro (б) and composite xerogel G4C4-Xero (в)

下载 (78KB)
索引源数据
5. Fig. 4. Human fibroblasts after 24 h of cultivation in a standard nutrient medium (а, б) and during co-cultivation with composite hydrogel G4C4-Hydro (в, г) and composite xerogel G4C4-Xero (д, е)

下载 (130KB)
索引源数据
6. Fig. 5. Proliferative activity of human fibroblasts after cultivation in a standard nutrient medium (control) and after co-cultivation with the composite hydrogel G4C4-Hydro and the composite xerogel G4C4-Xero

下载 (60KB)
索引源数据
7. Fig. 6. Human fibroblasts after 72 h of cultivation on plastic in a standard nutrient medium (a)and when cultivated on the surface of the composite hydrogel G4C4-Hydro (б) and the composite xerogel G4C4-Xero (в)

下载 (83KB)
索引源数据
8. Fig. 7. Human fibroblasts after 72 h of cultivation in inside the composite xerogel G4C4-Xero

下载 (102KB)
索引源数据

版权所有 © Russkiy Vrach Publishing House, 2025