Preparation of women with habitual early miscarriage for pregnancy, by using different doses of allogeneic lymphocytes


Cite item

Full Text

Abstract

The objective of the study was to optimize lymphocyte immunotherapy (LIT) and to evaluate its efficiency in patients with habitual pregnancy loss of alloimmune genesis during their preparation for pregnancy and in its first trimester. The study was conducted in 64 women of reproductive age, of them 26 patients received LIT in amount of 50 million cells, 18 patients and 20 had LIT in amount of 100 million and 150 million cells, respectively. There were reductions in the level of CD3-56+16+ cells and CD4/CD8 cell ratio, increases in the levels of CD8+ cells and IgM, a decrease in the response of female lymphocytes to phytohemagglutinin, and rises in the levels of blocking factors and antiparental antileukocyte antibodies. The use of 100 million and 150 million cells has been found to be most effective in the preparation of married couples with habitual early pregnancy losses of alloimmune genesis for pregnancy. This makes it possible to increase the level of blocking factors and the generation of antiparental antibodies and to reduce the level of natural killer (CD3-56+16+) cells in peripheral blood.

About the authors

L A Petrosyan

I. M. Sechenov Moscow Medical Academy

Email: pla2407hi@mail.ru
I. M. Sechenov Moscow Medical Academy

N K Matveyeva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Sciences, Moscow

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Sciences, Moscow

L V Krechetova

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Sciences, Moscow

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Sciences, Moscow

Ye L Golubeva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Sciences, Moscow

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Sciences, Moscow

M M Ziganshina

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Sciences, Moscow

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Sciences, Moscow

References

  1. Гнипова В. В. Оптимизация патогенетической терапии у женщин с привычным невынашиванием беременности I триместра на основании исследования состояния эндометрия: Автореф. дис. … канд. мед. наук. - М., 2003.
  2. Сидельникова В. М. Актуальные проблемы невынашивания беременности: Цикл клинических лекций. - М., 1999.
  3. Сидельникова В. М. Привычная потеря беременности. - М., 2002.
  4. Ширшев С. В. Механизмы иммунного контроля процессов репродукции. - Екатеринбург, 1999.
  5. Beer A. E., Kwak J. Reproductive Medicine: Program Finch University of Health Science. - Chicago, 2000.
  6. Clark D. A., Lea R. G., Podor T. et al. // Ann. N. Y. Acad. Sci. - 1991. - Vol. 656. - P. 524-536.
  7. Clover L. M., Sargent I. L., Townsend A. et al. // Eur. J. Immunol. - 1995. - Vol. 25, N 2. - P. 543-553.
  8. Coulam C. B., Kryza L. W., Bustillo M. // Hum. Reprod. - 1994. - Vol. 9. - P. 2265-2269.
  9. Coulam C. B., Stephenson M., Stern J. J., Clark D. A. // Am. J. Reprod. Immunol. - 1996. - Vol. 35, N 4. - P. 352-359.
  10. Coulam C. B., Clark D. A., Beer A. E. et al. // Am. J. Reprod. Immunol. - 1997. - Vol. 38, N 2. - P. 57-74.
  11. Darmochwal-Kolarz D., Leszczynska-Gorzelak B., Rolinski J., Oleszczuk J. // Eur. J. Obstetr. Gynecol. Reprod. Biol. - 1999. - Vol. 86, N 2. - P. 165-170.
  12. Emmer P. M., Nelen W. L., Steegers E. A. et al. // Hum. Reprod. - 2000. - Vol. 15, N 5. - P. 1163-1169.
  13. Gulan G., Podack E. R., Rukavina D. et al. // Am. J. Reprod. Immunol. - 1997. - Vol. 38, N 1. - P. 9-18.
  14. Kwak J. Y. // Am. J. Reprod. Immunol. - 1999. - Vol. 41, N 1. - P. 91-98.
  15. Lachapelle M. H., Miron P., Hemmings R., Roy D. C. // J. Immunol. - 1996. - Vol. 156, N 10. - P. 4027-4034.
  16. Lim K. J., Odukoya O. A., Ajjan R. A. et al. // Mol. Hum. Reprod. - 1998. - Vol. 4, N 1. - P. 77-81.
  17. Ober C., Rosinsky B., Grimsley C. et al. // J. Reprod. Immunol. - 1996. - Vol. 32, N 2. - P. 111-123.
  18. Reindollar R. // Obstetr. Gynecol. Clin. - 2000. - Vol. 27. - P. 3.
  19. Vassiliadou N., Bulmer J. N. // Hum. Reprod. - 1996. - Vol. 11, N 7. - P. 1569-1574.
  20. With N. D., Grassi K. M., Givan A. L. et al. // J. Immunol. - 1997. - Vol. 158, N 6. - P. 3017-3027.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2009 Bionika Media