Detection of herpes simplex virus in male sex cells in experimental infection of testicular organ culture and in the ejaculate in male fertility disorders


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Abstract

The use of a complex of methods (rapid culture method, polymerase chain reaction (PCR), in situ PCR) has revealed that the detection rate for herpes simplex virus (HSV) in the fraction of mobile spermatozoa is higher in patients with impaired fertility (20.4-26.4%) than in apparently healthy men (2.6-6.1%). An average of 1.5% of the mobile spermatozoa isolated from an infected ejaculate contains viral DNA. When assisted reproductive technologies are applied, the gradient centrifugation of the whole ejaculate makes it possible to wash the ejaculate from HSV or to reduce a viral load in 60% of cases. The development of an experimental model of herpetic infection in the murine testicular organ culture has ascertained that early spermatogenic cells, i.e. spermatogonia and spermatocytes, are susceptible to viral infestation.

About the authors

Viktor Alekseevich Naumenko

Email: virol18@mail.ru

R R Klimova

L F Kurilo

L V Shileyko

E V Chichev

Z S Gadzhieva

A S Tsibizov

Yu A Gavrilov

A A Kushch

G T Sukhikh

V A Naumenko

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

R R Klimova

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

L F Kurilo

Medical Genetics Research Center, Russian Academy of Medical Sciences

Medical Genetics Research Center, Russian Academy of Medical Sciences

L V Shileiko

Medical Genetics Research Center, Russian Academy of Medical Sciences

Medical Genetics Research Center, Russian Academy of Medical Sciences

Ye V Chichev

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

Z S Gadzhiyeva

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

A S Tsibizov

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

Yu A Gavrilov

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Technologies, Moscow

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Technologies, Moscow

A A Kushch

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

D.I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences

G T Sukhikh

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Technologies, Moscow

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Technologies, Moscow

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