CYTOKINE MRNA GENE EXPRESSION PROFILE IN THE VAGINAL SMEARS OF REPRODUCTIVE-AGE WOMEN WITH NONSPECIFIC VAGINITIS AND BACTERIAL VAGINOSIS


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Abstract

Objective. To study the cytokine mRNA gene expression profile in the vaginal discharge of patients with nonspecific vaginitis (NV) and bacterial vaginitis (BV). Subjects and methods. One hundred and twenty-six patients aged 19 to 45 years were examined. Cytokine mRNA gene expression was determined by real-time reverse transcription-polymerase chain reaction. Results. The patients with NV showed by a significant increase in the mRNa gene expression of the cytokines IL-1b, IL-6, IL-8, IL-10, TNF, IFNG, LIF, and СD45 and a reduction in the levels of IL-12a and IL-18 as compared to healthy women. Those with BV were found to have a significant rise in the mRNA gene expression of IL-6, IL-8, IL-10, and LIF and a decrease in the levels of IL-12a and IL-18 as compared to the control group. Conclusion. The following cytokines: IL-1b, IL-6, IL-8, IL-10, TNF, IFNG, LIF, IL-12a, IL-18, and СD45 are essential in NV and BV. The ratio of IL-10/IL-18 mRNA gene expression is an important diagnostic criterion for a vaginal dysbiotic process.

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About the authors

G. T SUKHIKH

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

D. Y TROFIMOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

O. V BURMENSKAYA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

G. R BAIMAROVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Email: bayramova@mail.ru

O. S NEPSHA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

A. E DONNIKOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

E. R DURINYAN

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

A. M BIRYUKOVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

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