POSSIBILITIES OF PREDICTING THE OUTCOMES OF ASSISTED REPRODUCTIVE TECHNOLOGIES FROM SPERM mRNA LEVELS


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Abstract

Objective. To determine a correlation between the expression of sperm protamine type 1 and 2 (PRM-1 and PRM-2), fertiline-β (ADAM-2) mRNA, and the outcomes of treatment for infertility in the use of assisted reproductive technologies (ART). Material and methods. Quantitative reverse-transcription polymerase chain reaction was used to study the PRM-1, PRM-2, and ADAM-2 gene mRNA profile in 79 married couples with different results of in vitro fertilization and embryo transfer ((IVF-ET) treatment for infertility. Of them, 13 couples had a successful outcome of the IVF and ET program; 66 couples had an unsuccessful outcome (10 couples with spontaneous miscarriage before 11 weeks gestation, 19 couples with biochemical pregnancy, and 37 couples with failed IVF-ET). The clinical pregnancy rate with reference to ET was 29.1% (23/79). Results. The expression of ADAM-2 mRNA was shown to be significantly decreased in the biochemical pregnancy and failed IVF groups as compared to the control one. In addition, that of PRM-1 and PRM-2 was less in the biochemical pregnancy group as compared to the control group. Conclusion. To study a role of sperm mRNA in the prediction of the outcome of ART programs is promising for the investigation of a parental contribution to embryo development. The poor prognosis of ART programs is associated with the decreased expression of the ADAM-2, PRM-1, and PRM-2 mRNA genes.

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About the authors

V. Yu SMOLNIKOVA

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

O. E KRASNOSHCHOKA

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Email: mydiss@mail.ru
Moscow

O. V BURMENSKAYA

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

D. Yu TROFIMOV

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

E. A KALININA

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

G. T SUKHIKH

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

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