THE COMPARATIVE LEVELS OF REGULATORY TRANSPORT PROTEINS, CYTOKINES, AND SPECIFIC IMMUNE COMPLEXES IN THE BLOOD, PERITONEAL FLUID, AND CYSTIC CONTENTS OF WOMEN WITH EXTERNAL ENDOMETRIOSIS
- Authors: ZORINA V.N1, TRETYAKOVA T.V2, ZORINA R.M1, BAZHENOVA L.G3, RYABICHEVA T.G4, ZORIN N.A1
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Affiliations:
- Research Laboratory of Immunology, Novokuznetzk State Institute for Postgraduate Training of Physicians, Ministry of Health and Social Development of the Russian Federation
- OOO MEDIKA Medical Center, Novokuznetzk
- Novokuznetzk State Institute for Postgraduate Training of Physicians, Ministry of Health and Social Development of the Russian Federation
- ZAO Vektor-Best, Koltsovo
- Issue: No 6 (2012)
- Pages: 28-32
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/246785
- ID: 246785
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Abstract
Objective. To comparatively study the blood, peritoneal fluid (PF), and cystic contents (CC) levels of a number of cytokines (IL-6, IL-8, TNF-α), alpha2-macroglobulin (a2-MG), pregnancy-associated alpha2-glycoprotein (a2-PAG), lactoferrin (LF), and alphal-antitrypsin (a1-AT), as well as the concentrations of circulating immune complexes of class G antibodies with a2-MG and a2-PAG in Stage III external genital endometriosis to specify their role in the pathogenesis of the disease and the possibility of their use in differential diagnosis. Subjects and methods. A group of 48 women (33.2±1.8 years of age) diagnosed as having Stage III external genital endometriosis was examined. Sera from 35 apparently healthy women (38.20±2.06 years of age) selected according to the results of planned prophylactic medical examination and sera, PF, and CC from 10 women (41.9±6.5 years of age) with simple ovarian serous cysts (SOSC) were studied as control and comparison groups, respectively. The content of a2-MG, a2-PAG, and al-AT was measured by quantitative rocket immunoelectrophoresis. The levels of IL-6, TNF-a, IL-8, LF, a2-MG-IgG, and a2-PAG-IgG were examined using solid-phase enzyme immunoassay (EIA). Results. The patients with endometriosis were found to have higher levels of the immune complexes of a2-PA G-IgG, LF, al-AT, and TNF-a and a lower concentration of a2-MG than did the healthy individuals (only the a2-MG concentration was decreased in ovarian retention cyst (ORC). In endometriosis, PF showed higher a2-PAG-IgG and IL-6 levels and a lower LF concentration than in ORS. In this condition, CC exhibited increases in all the study indicators as compared with ORS. Conclusion. The found changes are indicative of the more magnitude of alterations in humoral immunity and more aggressive processes in endometriosis and may be used as additional diagnostic and prognostic criteria in the clinical practice of an obstetrician/gynecologist.
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About the authors
V. N ZORINA
Research Laboratory of Immunology, Novokuznetzk State Institute for Postgraduate Training of Physicians, Ministry of Health and Social Development of the Russian Federation
Email: macroglobulin@yandex.ru
T. V TRETYAKOVA
OOO MEDIKA Medical Center, Novokuznetzk
Email: ptv6l@yandex.ru
R. M ZORINA
Research Laboratory of Immunology, Novokuznetzk State Institute for Postgraduate Training of Physicians, Ministry of Health and Social Development of the Russian Federation
Email: macroglobulin@yandex.ru
L. G BAZHENOVA
Novokuznetzk State Institute for Postgraduate Training of Physicians, Ministry of Health and Social Development of the Russian Federation
Email: L_Bagenova@mail.ru
T. G RYABICHEVA
ZAO Vektor-Best, Koltsovo
N. A ZORIN
Research Laboratory of Immunology, Novokuznetzk State Institute for Postgraduate Training of Physicians, Ministry of Health and Social Development of the Russian Federation
Email: macroglobulin@yandex.ru
References
- Адамян Л.В., Бургова Е.Н., Осипова А.А., Сомова М.М. Роль лактоферрина перитонеальной жидкости при генитальном эндометриозе // Современные технологии в диагностике и лечении гинекологических заболеваний: Сборник трудов. - М., 2007. - С. 213-214.
- Адамян Л.В., Бургова Е.Н., Сомова М.М. и др. Окислительный стресс и генитальный эндометриоз // Пробл. репрод. - 2008. - № 4. - С. 6-9.
- Аксельсен Н., Крелль Й., Веке Б. Руководство по количественному иммуноэлектрофорезу. - М.: Мир, 1977.
- Баженова Л.Г., Зорина В.Н., Промзелева Н.В. и др. Концентрация альфа-2-макроглобулина, иммуноглобулина G и их комплексов в крови, кистозном содержимом и перитонеальной жидкости при опухолях яичников у женщин // Рос. вестн. акуш.- гин. -2005. - Т. 5, № 3. - С.10-13.
- Зорина В.Н., Зорина Р.М., Левченко В.Г. и др. Концентрация комплексов макроглобулин - IgG, как возможный критерий для прогнозирования гестоза и оценки степени его тяжести // Клин. лаб. диагн. - 2001. - № 8. - С. 48-49.
- Меньшикова Е.Б. Окислительный стресс. Прооксиданты и антиоксиданты. - М.: Слово, 2006.
- Gmyrek G.B., Sieradzka U., Goluda M. et al. Flow cytometric évaluation of intracellular cytokine synthesis in peripheral mononuclear cells of women with endometriosis // Immunol. Invest. - 2008. - Vol. 37, № 1. - P. 43-61.
- Iborra A., Palacio J.R., Martinez P. Oxidative stress and autoimmune response in the infertile woman // Chem. Immunol. Allergy. - 2005. - Vol. 88. - P. 150-162.
- Lambrinoudaki I.V., Augoulea A., Christodoulakos G.E. et al. Measurable serum markers of oxidative stress response in women with endometriosis // Fertil. Steril. - 2009. - Vol. 91, №1. -P. 46-50.
- Legrand D., Elass E., Carpenter M., Mazurer J. Lactoferrin: a modulator of immune and inflammatory responses // Cell. Mol. Life Sci. - 2005. - Vol. 62. - P. 2549-2559.
- Mihalyi A., Gevaert O., Kyama C.M. et al. Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers // Hum. Reprod. - 2010. - Vol. 25, № 3. -P. 654-664.
- Nishida M., Nasu K., Narahara H. Role of chemokines in the pathogenesis of endometriosis // Front. Biosci. (Schol Ed). - 2011. - Vol. 3. -P. 1196-1204.
- Podgaec S., Abrao M.S., Dias J.A. Jr. et al. Endometriosis: an inflammatory disease with a Th2 immune response component // Hum. Reprod. - 2007. - Vol. 22, № 5. - P.1373-1379.
- Seeber B., Sammel M.D., Fan X. et al. Panel of markers can accurately predict endometriosis in a subset of patients // Fertil. Steril. - 2008. - Vol. 89, № 5. - P. 1073-1081.
- Velasco I., Acien P., Campos A. et al. Interleukin-6 and other soluble factors in peritoneal fluid and endometriomas and their relation to pain and aromatase expression // J. Reprod. Immunol. - 2010. - Vol. 84, № 2. -P. 199-205.
- Wu S.M., Patel D.D., Pizzo S.V. Oxidized a2-macroglobulin (alpha 2M) differentially regulates receptor binding by cytokines/growth factors: implications for tissue injury and repair mechanisms in inflammation // J. Immunol. - 1998. -Vol. 161, № 8. - P. 4356-4365.
- Zorin N.A., Zorina V.N., Zorina R.M. Role of proteins of the macroglobulin family in regulation of tumor growth // Russ. J. Develop. Biol. - 2006. - Vol. 37, № 1. - P. 12-19.
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