COARSE-GRAINED OOCYTE CYTOPLASMIC DESTRUCTION IN AN IN VITRO FERTILIZATION CYCLE: ULTRASTRUCTURAL ANALYSIS
- Authors: MAKAROVA N.P1, KAZARYAN L.M1, KALININA E.A1, BARANOVA G.B2, POLYAKOV V.Y.2
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Affiliations:
- Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia
- All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences
- Issue: No 8-2 (2012)
- Pages: 103-106
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/246871
- ID: 246871
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Abstract
Objective. To analyze the ultrastructural pattern of the oocytes with coarse-grained cytoplasm destruction, obtained during infertility treatment by in vitro fertilization (IVF). Subjects and methods. A 31-year-old patient had suffered from primary tuboperitoneal infertility for 5 years. Short stimulation protocol yielded 11 oocytes, of which 2 were in MII stage, 6 in MI, and 3 in GV. All the oocytes had marked central granularity. Only one mature oocyte was fertilized by ICSI; however, zygotic fission was absent. Immature oocytes were fixed on the day of aspiration and sent for ultrastructural study. Results. An electron microscopic examination of oocytes revealed destructive changes in the cytoplasmic components: clustering of cellular organelles; swelling of smooth endoplastic reticulum cisterns; disappearance of mitochondria and their vacuolization. Conclusions. The observed ultrastructural changes in the oocyte may reflect destructive processes associated with imminent apoptosis in which further embryonic development of the cell is impossible regardless of occurred insemination.
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About the authors
N. P MAKAROVA
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia
Email: np.makarova@gmail.com
L. M KAZARYAN
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia
Email: l_kazaryan@oparina4.ru
E. A KALININA
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia
Email: e_kalinina@oparina4.ru
G. B BARANOVA
All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences
Email: salex42@mail.ru
V. Yu POLYAKOV
All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences
Email: polyakovvladimir@mail.ru
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