NOVELTY ABOUT EMBRYO IMPLANTATION INTO THE UTERINE ENDOTHELIUM

Successful in vitro fertilization (IVF) and embryo transfer into the uterine cavity do not always result in desired pregnancy. Novel methods for enhancing the efficiency of IVF are mainly sought among high technologies. However, the success of ongoing medical studies and maneuvers frequently depends not only on the application of high technologies, but also on that of simple methods and procedures, which are to be studied and used in practice. The authors developed and tested a new procedure to increase the possibility of successful embryo implantation to the uterine cavity (patent for invention No. 2444315) and an embryo implanter (patent for useful model No. 106521). According to the authors’ procedure, the technical result in improving the implantation of blastocysts to the endometrium is achieved by creating the minimal air pressure in the uterine cavity by means of a gas mixture (CO 2 + N), which gives rise to the closest contact with and adhesion of blastocysts to the endometrium, and to subsequent implantation. In the initial phase of the investigation, it was very interesting for the authors to test the effect of a gas mixture of 6% CO 2 and 94% N on oocyte viability. For this, the immature oocytes of 30 patients, which were unfit for fertilization and should be disposed, were examined. The findings showed that the viability of oocytes exposed to the gas mixture of CO 2 and N remained unchanged in all cases. The gas mixture was concurrently examined for its effect on sperm motility and viability. The total motility (a + b%) of fast progressive and slow progressively spermatozoids in the ejaculate samples incubated in IVF medium in a thermostat, by adding the experimental gas mixture (CO 2 + N) was ascertained to increase by 8.3 and 7.3%, respectively, as compared in the samples incubated under the same conditions, but without adding the gas mixture 5.5 and 3.7%).

in vitro fertilization, spermatozoids, implantation, carbon dioxide, nitrogen