In vitro cytokine production by peripheral blood mononuclear cells in patients with recurrent miscarriage during alloimmunization


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Abstract

Objective. To study Th1 and Th2 cytokine production by mitogen-stimulated peripheral blood mononuclear cells from patients with recurrent miscarriage (RM) during alloimmunization in their pregestational preparation and in the first trimester of pregnancy resulting in a live baby. Subject and methods. The levels of cytokines were estimated by flow cytometry using multiplex analysis in 20 patients with RM. Results. The time course of changes in Th1/Th2 balance during alloimmunization includes the formation of a proinflammatory background preceding the initiation of pregnancy and a further Th2 profile that ensures pregnancy prolongation. Conclusion. To monitor Th1/Th2 responses during alloimmunization may serve as the basis for understanding the role of the immune system in the regulation of reproduction and for enhancing the efficiency of RM treatment.

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About the authors

L. V Krechetova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: k_l_v_@mail.ru
PhD in medical sciences, Head of Laboratory of Clinical Immunology

M. A Nikolaeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology

L. V Vanko

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: LVanko@oparina4.ru
MD, Professor, Leading researcher, Laboratory of Clinical Immunology

M. M Ziganshina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: mmz@mail.ru
junior researcher in Laboratory of Clinical Immunology

V. V Vtorushina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: vtorushina@inbox.ru
PhD in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

N. A Khachatryan

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nelly1986@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

N. K Tetruashvili

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: tetrauly@mail.ru
MD, Head of the Department of Pregnancy Loss Prevention and Therapy

G. T Sukhikh

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: gtsukhikh@mail.ru
MD, Professor, Academician of Russian Academy of Medical Sciences, Director

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