ANALYSIS OF THE RESULTS OF USING A REAL-TIME POLYMERASE CHAIN REACTION ASSAY FOR THE DIAGNOSIS OF ACUTE RESPIRATORY DISEASES AND INFLUENZA IN PREGNANT WOMEN AND NEONATES IN THE PERIOD OF EPIDEMICS


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Abstract

Objective. To determine the capabilities of a real-time polymerase chain reaction (PCR) assay in diagnosing ARVIs, including respiratory syncytial viral infection and influenza, in pregnant women and neonates. Subject and methods. The trial enrolled 174 adult patients, including 113 pregnant patients and 61 gynecological patients, with a referral diagnosis of ARVI or influenza and 52 infants, including premature babies. A molecular genetic study of biological materials obtained from the nose and pharynx, as well as buccal scrapes and tracheal aspirates was performed by real-time PCR. Results. Among all the patients, the proportion of positive PCR assay results was 32.2%; at the same the assay revealed genetic material of influenza virus in 10.9%. In the neonatal infants, human respiratory syncytial virus (HRSV) was the leading etiological viral agent causing a severe respiratory tract lesion. The findings suggest that the prediagnosis supposing the viral nature of the disease is much more commonly confirmed in nonpregnant patients, which reflects the dormant clinical course of respiratory infection in pregnant women. Emphasis is laid on the leading role of respiratory syncytial virus in the common etiological pattern of viral infections recorded among the neonatal unit patients and on the special severity of this infection. Conclusion. PCR could establish the etiological diagnosis and use the most effective antiviral therapy within the first 24-48 hours after the disease onset,

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About the authors

N. V SHARASHKINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: sharashkina@inbox.ru
Candidate of Medical Sciences, therapist, Department of Therapeutics 117997, Russia, Moscow, Ac. Oparina Str. 4

I. V NIKITINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: i_nikitina@oparina4.ru
PhD, doctor of the intensive care department of neonatology and pediatrics 117997, Russia, Moscow, Ac. Oparina Str. 4

O. V BURMENSKAYA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_bourmenskaya@oparina4.ru
PhD, staff scientist of laboratory of molecular genetic methods 117997, Russia, Moscow, Ac. Oparina Str. 4

O. S NEPSHA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_nepsha@oparina4.ru
PhD, staff scientist of laboratory of molecular genetic methods 117997, Russia, Moscow, Ac. Oparina Str. 4

A. E DONNIKOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
PhD, senior staff scientist of laboratory of molecular genetic methods 117997, Russia, Moscow, Ac. Oparina Str. 4

D. Yu TROFIMOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: d_troflmov@oparina4.ru
PhD, Head of the of laboratory of molecular genetic methods 117997, Russia, Moscow, Ac. Oparina Str. 4

N. K RUNIKHINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_runihina@oparina4.ru
MD, Professor, Head of the Therapeutic Department 117997, Russia, Moscow, Ac. Oparina Str. 4

O. V IONOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_ionov@oparina4.ru
Ph.D., Head of the intensive care department of neonatology and pediatrics 117997, Russia, Moscow, Ac. Oparina Str. 4

N. E KAN

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_kan@oparina4.ru
MD, head of the obstetric observation department 117997, Russia, Moscow, Ac. Oparina Str. 4

V. L TYUTYUNNIK

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tyutyunnik@mail.ru
MD, the head of Academician 117997, Russia, Moscow, Ac. Oparina Str. 4

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