Estimation of cytokine profile during chemosensitized blood photomodification in patients with a history of recurrent miscarriage of viral genesis


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Abstract

Objective. To analyze the expression of the tumor necrosis factor-а (TNF-а) gene in blood mononuclear cells and the production of cytokines (TNF-а, interferon-а (IFN-а), and transforming growth factor в 1 (TGF-fi 1)) in the serum of patients with herpesvirus infection and a history of recurrent miscarriage, who underwent chemosensitized blood photomodification (BPM). Subject and methods. Comprehensive examination and treatment were performed in 74 patients with herpesvirus infection, including 36 women with no history of recurrent miscarriage (Group 1) and 38 with a history of this condition (Group 2). TNF-а gene expression, cytokine profile, and a change in specific antibody titers against herpes simplex virus (HSV) type 2 and cytomegalovirus were estimated in all the patients before, during, and after therapy. Results. Examination of TNF-а gene expression revealed that 100% of Group 1 patients showed a significant downward trend in this indicator during therapy. Lower TNF-а production was noted in 60% of the patients in Group 1. In Group 2, there was a significant trend in declining TNF-а gene expression and a reduction in TNF-а production 10 days after treatment initiation was detected in 62.5% of the patients. There was a significant increase in the level of TGF-Pj in Group 1 patients during therapy. In Group 2, there was a mediated elevation in TGF-fi 1 levels by the completion of an 18-20-day therapy cycle, which is a positive effect and promotes suppressed HSV replication. BPM failed to affect serum IFN-а levels; no changes in specific antibody titers were found in the examined groups. Conclusion. Thus, chemosensitzed BPM has a positive impact in all the patients of the examined groups: the mechanisms of the body’s antiviral protection activate, which will be able further to increase the rates of conception and a favorable pregnancy outcome in women with recurrent miscarriage of infection genesis.

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About the authors

O. V Makarov

N.I. Pirogov National Research Medical University, Ministry of Health of Russia

MD, Professor, Head of Obstetrics and Gynecology Department One, Faculty of Therapeutics

A. Z Khashukoeva

N.I. Pirogov National Research Medical University, Ministry of Health of Russia

Email: azk05@mail.ru
MD, Professor of Obstetrics and Gynecology Department One, Faculty of Therapeutics

O. A Svitich

I.I. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences

Email: svitichoa@yandex.ru
MD, Head of the Laboratory of Molecular Immunology

E. A Markova

N.I. Pirogov National Research Medical University, Ministry of Health of Russia

Email: markova.eleonora@mail.ru
post-graduate of Obstetrics and Gynecology Department One, Faculty of Therapeutics

S. A Khlynova

N.I. Pirogov National Research Medical University, Ministry of Health of Russia

Email: doc-khlinova@mail.ru
PhD, associate professor of Obstetrics and Gynecology Department One, Faculty of Therapeutics

References

  1. Сидельникова В.М. Подготовка и ведение беременности у женщин с привычным невынашиванием. Методические пособия и клинические протоколы. М.: МЕДпресс-информ; 2011. 219 с. [Sidelnikova V.M. Preparation and maintenance of pregnancy in women with recurrent pregnancy loss. Manuals and clinical protocols. M. MEDpress Inform; 2011. 219 p.]
  2. Гаджиева Ф.Г. Цитокины как патогенетические маркеры воспалительного процесса при невынашивании беременности инфекционного генеза. Проблемы репродукции. 2011; 17(1): 110-3. [Cadzhieva F.G. Cytokines as pathogenic markers of the inflammatory process in miscarriage of infectious origin. Problems of reproduction. 2011; 17 (1): 110-3.]
  3. Сухих Г.Т., Ванько Л.В. Генитальный герпес. М.: Миклош; 2010. 343 с. [Sukhikh G/Г., Vanko L.V. Genital herpes. M. Miklosh; 2010. 343 p.]
  4. Макаров О.В., Озолиня Л.А., Сумеди Т.Н. Изменение показателей иммунитета у пациенток с невынашиванием беременности инфекционного генеза в I триместре. Российский вестник акушера-гинеколога. 2008; 6: 28-32. [Makarov O.V., Ozolinya L.A., Sumedi LN. Changes in the parameters of immunity in patients with miscarriage of infectious origin in the 1st trimester. Russian Obstetrician Bulletin. 2008; 6: 28-32.]
  5. Попова А.Ф., Пастушенков В.Л. Клинические и лабораторные аспекты герпетической инфекции у пациенток c невынашиванием беременности. Журнал акушерства и женских болезней. 2010; 59(6): 58-68. [Popova A.F., Pastushenkov V.L. Clinical and laboratory aspects of herpes infection in patients with miscarriage. Journal of Obstetrics and women’s diseases. 2010; 59 (6): 58-68.]
  6. Шульженко А.Е., Зуйкова И.Н. Подходы к иммунотерапии рецидивирующего простого герпеса. Эффективная фармакотерапия в акушерстве и гинекологии. 2010; 3: 10-5. [Shulzhenko A.E., Zuikova I.N. Approaches to immunotherapy of recurrent herpes simplex. Effective pharmacotherapy in obstetrics and gynecology. 2010; 3: 10-5.]
  7. Ершов Ф.И. Антивирусные препараты. Справочник. 2-е изд. М.: ГЭОТАР-Медиа; 2006. 311 с. [Ershov F.I. Antiviral drugs. Handbook. 2nd ed. M.: GEOLAR Media; 2006. 311 p.]
  8. Хашукоева А.З., Свитич О.А., Маркова Э.А., Отдельнова О.Б., Хлынова С.А. Фотодинамическая терапия - противовирусная терапия? История вопроса. Перспективы применения. Лазерная медицина. 2012; 16(2): 63-7. [Hashukoeva A.Z., Svitich O.A., Markova E.A., Otdelnova O.B., Khlynova S.A. Photodynamic therapy - antiviral therapy? Background. Application prospects. Laser medicine. 2012; 16 (2): 63-7.]
  9. Гейниц А.В., Сорокатый А.Е., Ягудаев Д.М., Трухманов Р.С. Фотодинамическая терапия. История создания метода и ее механизмы. Лазерная медицина. 2007; 11(3): 42-6. [Geynits A.V., Sorokaty A.E., Yagudaev D.M., Trukhmanov R.S. Photodynamic therapy. History of the method and its mechanisms. Laser medicine. 2007; 11 (3): 42-6.]
  10. Квачева З.Б., Лобанок Е.С., Вотяков В.И., Шуканова Н.А., Воробей А.В., Николаева С.Н., Титов Л.П. Фотодинамическое ингибирование инфекции, вызванной вирусом простого герпеса типа 1 в культуре клеток, при использовании порфиринов, индуцируемых 5-аминолевулиновой кислотой. Вопросы вирусологии. 2005; 50(4): 44-7. [Kvacheva Z.B., Lobanok E.S., Votyakov V.I., Shukanova N.A., Vorobey A.V., Nikolaeva S.N., Titov L.P. Photodynamic inhibition of infection caused by herpes simplex virus type 1 in cell culture using porphyrins induced by 5-aminolevulinic acid. Problems of Virology. 2005; 50 (4): 44-7.]
  11. Бахарева И.В., Ганковская Л.В., Ковальчук Л.В., Свитич О.А., Романовская В.В., Кузнецов П.А., Магомедова А.М., Дворецкая Е.В. Экспрессия генов молекул врожденного иммунитета (TLR2, TLR4, HBD1) при беременности с высоким риском реализации внутриутробной инфекции. Лечение и профилактика. 2012; 1: 44-50. [Bakhareva I.V., Gankovskaya L.V., Kovalchuk L.V., Svitich O.A., Romanovskaya V.V., Kuznetsov P.A., Magomedova A.M., Dvoretskaya E.V Expression of gene molecules of innate immunity (TLR2, TLR4, HBD1) during pregnancy at high risk of intrauterine infection. Treatment and prevention. 2012; 1: 44-50.]
  12. Соснова Е.А. Предгравидарная подготовка пациенток с вирусными инфекциями. Вопросы гинекологии, акушерства и перинатологии. 2011; 10(5): 72-8. [Sosnova E.A. Pregravid preparation of patients with viral infections. Questions of gynecology, obstetrics and perinatology. 2011; 10 (5): 72-8.]
  13. Carpentier P.A., Dingman A.L., Palmer T.D. Placental TNF-а signaling in illness-induced complications of pregnancy. Am. J. Pathol. 2011; 178(6): 2802-10.
  14. Baron S., Tyring S.K., Fleischmann W.R. Jr., CoppenhaverD.H., NieselD.W., Klimpel G.R. et al. The interferons. Mechanisms of action and clinical applications. JAMA. 1991; 266(10): 1375-83.
  15. Aboagye-Mathiesen G, Toth F.D., Zdravkovic M., Ebbesen P. Human trophoblast interferons: production and possible roles in early pregnancy. Early Pregnancy. 1995; 1(1): 41-53.
  16. Skrzypczak J., Wirstlein P., Mikolajczyk M., Ludwikowski G., Zak T. TGF superfamily and MMP2, MMP9, TIMP1 genes expression in the endometrium of women with impaired reproduction. Folia Histochem. Cytobiol. 2007; 45(Suppl. 1): S143-8.
  17. Zhao F.X., Zhang Y.Y., Liu R.H., Li S.M. Effect of blockage of costimulatory signal on murine abortion-prone model. Chinese Med. J. 2007; 120(14): 1247-50.
  18. Макаров О.В., Ковальчук Л.В., Ганковская Л.В., Бахарева И.В., Ганковская О.А. Невынашивание беременности и врожденный иммунитет. М.: ГЭОТАР- Медиа; 2007. 175 с. [Makarov O.V., Kovalchuk L.V., Gankovskaya L.V., Bakhareva I.V., Gankovskaya O.A. Miscarriage and innate immunity. M.: GEOTAR Media; 2007. 175 p.]
  19. Ковальчук Л.В., Игнатьева Г.А., Ганковская Л.В. Иммунология. Практикум. Учебное пособие. М.: ГЭОТАР-Медиа; 2012. 174 с. [Kovalchuk L.V., Ignatieva G.A., Gankovskaya L.V. Immunology. Workshop. Textbook. M.: GEOTAR Media; 2012. 174 p.]
  20. Ганковская О.А., Бахарева И.В. , Ганковская Л.В., Сомова О.Ю., Зверев В.В. Исследование экспрессии генов TLR9, NF-kB, ФНОа в клетках слизистой цервикального канала беременных с герпесвирусной инфекцией. Журнал микробиологии, эпидемиологии и иммунобиологии. 2009; 2: 61-5. [Gankovskaya O.A., Bakhareva I.V., Gankovskaya L.V., Somova O.Yu., Zverev V.V. Study of expression of genes TLR9, NF-kB, TNF in mucosal cells of pregnant women with cervical herpes infection. Journal of Epidemiology and Microbiology, Immunobiology. 2009; 2: 61-5.]
  21. Гланц С. Медико-биологическая статистика. М.: Практика; 1999. 462 с. [Glants S. Biomedical Statistics. M.: Practice; 1999. 462 p.]
  22. Гейдарова Н.Ф. Оценка метаболического резерва фагоцитов при привычном невынашивании у беременных с энтеровирусной инфекцией. Фундаментальные исследования. 2012; 7(ч.1): 66-70. [Geydarova N.F. Evaluation of phagocytes metabolic reserve in habitual miscarriage in pregnant patients with enterovirus infection. Fundamental research. 2012; 7 (Part 1): 66-70.]
  23. Сидельникова В.М. Привычная потеря беременности. М.: Триада-Х; 2005: 7-10, 64-7. [Sidelnikova V.M. Habitual pregnancy loss. M.: Triad-X; 2005: 7-10, 64-7.]
  24. Ершов Ф.И. Система интерферонов в норме и при патологии. М.: Медицина; 1996. 239 с. [Ershov F.I. Interferon system in health and disease. M.: Medicine; 1996. 239 p.]
  25. Кубанова А.А., Бутарева М.М., Саватеева М.В., Маркушева Л.И. Динамика трансформирующего фактора роста-b при УФ-терапии с длиной волны 311 нм у больных псориазом. Вестник дерматологии и венерологии. 2006; 5: 53-5. [Kubanova A.A., Butareva M.M., Savateeva M.V., Markusheva L.I. Dynamics of transforming growth factor-b during UV treatment with a wavelength of 311 nm in patients with psoriasis. Journal of Dermatology and Venereology. 2006; 5: 53-5.]
  26. Ball E., Robson S.C., Ayis S., Lyall F., Bulmer J.N. Expression of TGF beta in the placental bed is not altered in sporadic miscarriage. Placenta. 2007; 28(8-9): 965-71.
  27. Gao M.Z., Zhao X.M., Lin Y., Sun Z.G., Zhang H.Q. Effects of EG-VEGF, VEGF and TGF-^1 on pregnancy outcome in patients undergoing IVF-ET treatment. J. Assist. Reprod. Genet. 2012; 29(10): 1091-6.

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