Specific features of peripheral blood lymphocyte phenotype in women with recurrent miscarriage
- Authors: Krechetova L.V.1, Khachatryan N.A.1, Tetruashvili N.K.1, Vtorushina V.V.1, Stepanova E.O.1, Nikolaeva M.A.1, Sukhikh G.T.1
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Affiliations:
- Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
- Issue: No 10 (2014)
- Pages: 27-32
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/247249
- ID: 247249
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Open Access
Access granted
Abstract
Objective. To study peripheral blood lymphocyte subpopulations in women with recurrent miscarriage of alloimmune genesis, by estimating the counts of natural killer cells with a different phenotype, CD200 molecule, and natural regulatory T cells. Subject and methods. The phenotype of peripheral blood lymphocytes was determined on 18-20 days of the menstrual cycle in 70 patients with recurrent miscarriage after ruling out its known causes and in 15 fertile healthy women in a control group. Results. The married couples with recurrent miscarriage of alloimmune genesis constitute 21.9% of all married couples with recurrent pregnancy losses. The women with recurrent miscarriage had significantly higher levels of cells with the phenotype CD3 -CD8+, CD56+, CD3+CD56,16 +, CD3 -CD56,16 +, SCD56,16 +, CD5+CD19+, CD200 v, significantly lower counts of cells with the phenotype CD4 +CD25 highCD127 low/-, and a significantly higher ratio of cells with the phenotype CD20(+ and CD4+CD25 hig hCD127 low/-. Conclusion. The patients with recurrent miscarriage of alloimmune genesis versus the fertile women commonly show significant changes in the lymphocyte subpopulations of natural killer cells with a different phenotype and in the count of natural regulatory T cells and lymphocytes with the phenotype CD200+. The potential role of these changes in the diagnosis and monitoring of the efficiency of performed therapy is to be further investigated.
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About the authors
Lyubov V. Krechetova
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: l_krechetova@oparina4.ru
PhD in medical sciences, Head of Laboratory of Clinical Immunology
Nelly A. Khachatryan
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: nellyl986@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy
Nana K. Tetruashvili
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: tetrauly@mail.ru
MD, Head of the Department of Pregnancy Loss Prevention and Therapy
Valentina V. Vtorushina
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: vtorushina@inbox.ru
PhD in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology
Elena Olegovna Stepanova
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
Junior researcher, Laboratory of Clinical Immunology
Marina A. Nikolaeva
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology
Gennady T. Sukhikh
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: gtsukhikh@mail.ru
MD, Professor, Academician of Russian Academy of Sciences, Director
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